Anticonvulsant Effects of Fractions Isolated from Dinoponera quadriceps (Kempt) Ant Venom (Formicidae: Ponerinae)

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dc.citation.issue1
dc.citation.volume9
dc.contributor.authorMorais Ferreira Noga, Diana Aline
dc.contributor.authorMateus Brandao, Luiz Eduardo
dc.contributor.authorCagni, Fernanda Carvalho
dc.contributor.authorSilva, Delano
dc.contributor.authorOliveira de Azevedo, Dina Lilia
dc.contributor.authorAraujo, Arrilton
dc.contributor.authorSantos, Wagner Ferreira dos
dc.contributor.authorMiranda, Antonio [UNIFESP]
dc.contributor.authorSilva, Regina Helena da [UNIFESP]
dc.contributor.authorRibeiro, Alessandra Mussi [UNIFESP]
dc.coverageBasel
dc.date.accessioned2020-07-31T12:46:56Z
dc.date.available2020-07-31T12:46:56Z
dc.date.issued2017
dc.description.abstractNatural products, sources of new pharmacological substances, have large chemical diversity and architectural complexity. In this context, some toxins obtained from invertebrate venoms have anticonvulsant effects. Epilepsy is a neurological disorder that affects about 65 million people worldwide, and approximately 30% of cases are resistant to pharmacological treatment. Previous studies from our group show that the denatured venom of the ant Dinoponera quadriceps (Kempt) protects mice against bicuculline (BIC)-induced seizures and death. The aim of this study was to investigate the anticonvulsant activity of compounds isolated from D. quadriceps venom against seizures induced by BIC in mice. Crude venom was fractionated by high-performance liquid chromatography (HPLC) resulting in six fractions referred to as DqTx1-DqTx6. A liquid chromatography-mass spectrometry (LC/MS) analysis revealed a major 431 Da compound in fractions DqTx1 and DqTx2. Fractions DqTx3 and DqTx4 showed a compound of 2451 Da and DqTx5 revealed a 2436 Da compound. Furthermore, the DqTx6 fraction exhibited a major component with a molecular weight of 13,196 Da. Each fraction (1 mg/mL) was microinjected into the lateral ventricle of mice, and the animals were observed in an open field. We did not observe behavioral alterations when the fractions were given alone. Conversely, when the fractions were microinjected 20 min prior to the administration of BIC (21.6 nM), DqTx1, DqTx4, and DqTx6 fractions increased the latency for onset of tonic-clonic seizures. Moreover, all fractions, except DqTx5, increased latency to death. The more relevant result was obtained with the DqTx6 fraction, which protected 62.5% of the animals against tonic-clonic seizures. Furthermore, this fraction protected 100% of the animals from seizure episodes followed by death. Taken together, these findings indicate that compounds from ant venom might be a potential source of new anticonvulsants molecules.en
dc.description.affiliationUniv Fed Rio Grande do Norte, Dept Physiol, BR-59078970 Natal, RN, Brazil
dc.description.affiliationUniv Sao Paulo, Dept Biol, BR-14040901 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biophys, BR-04023062 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Pharmacol, BR-04023062 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biosci, BR-11015020 Sao Paulo, SP, Brazil
dc.description.affiliationUnifespBiophysics Department, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP 04023-062, Brazil
dc.description.affiliationUnifespPharmacology Department, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP 04023-062, Brazil
dc.description.affiliationUnifespBiosciences Department, Universidade Federal de São Paulo (UNIFESP), Santos, SP 11015-020, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipPró-reitoria de Pesquisa da Universidade Federal do Rio Grande do Norte (PROPESQ/UFRN)
dc.description.sponsorshipFundaçao de Apoio à Pesquisa do Estado do Rio Grande do Norte (FAPERN)
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.3390/toxins9010005
dc.identifier.citationToxins. Basel, v. 9, n. 1, p. -, 2017.
dc.identifier.doi10.3390/toxins9010005
dc.identifier.fileWOS000392980000005.pdf
dc.identifier.issn2072-6651
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56469
dc.identifier.wosWOS:000392980000005
dc.language.isoeng
dc.publisherMdpi Ag
dc.relation.ispartofToxins
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectant venomen
dc.subjectneuroactive compoundsen
dc.subjectbicucullineen
dc.subjecttonic-clonic seizuresen
dc.subjectpeptide fractionen
dc.subjectnatural producten
dc.titleAnticonvulsant Effects of Fractions Isolated from Dinoponera quadriceps (Kempt) Ant Venom (Formicidae: Ponerinae)en
dc.typeinfo:eu-repo/semantics/article
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