Sperm proteomics: potential impact on male infertility treatment

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dc.citation.issue3
dc.citation.volume13
dc.contributor.authorAgarwal, Ashok
dc.contributor.authorBertolla, Ricardo Pimenta [UNIFESP]
dc.contributor.authorSamanta, Luna
dc.coverageAbingdon
dc.date.accessioned2020-08-21T16:59:55Z
dc.date.available2020-08-21T16:59:55Z
dc.date.issued2016
dc.description.abstractSpermatozoa are unique cells that have highly compact DNA, motility (and hypermotility) patterns, a specific morphology, localized mitochondria and an apical acrosome. They are the end product of a dynamic process termed spermatogenesis. Sperm are therefore produced with specific proteins in order to effect different traits, such as the presence of cysteine-rich protamines in DNA, which effectively compacts DNA. Moreover, specific proteins are transferred during epididymal maturation and after ejaculation in order to render sperm capable of undergoing post-ejaculatory alterations, generally termed capacitation, which confers capacity to fertilize a mature oocyte. In addition, sperm exhibit several post-translational modifications, which are fundamental to their function, such as SUMOylation and ubiquitination. Discussed in this review is the current knowledge of the sperm proteome in terms of its composition and the function that these proteins determine, as well as their post-translational modifications and how these alter sperm functional integrity. Studies are emphasized that focus on shotgun proteomics - untargeted determination of the protein constituent of a cell in a given biological condition - and technologies currently applied toward that end are reviewed.en
dc.description.affiliationCleveland Clin, Amer Ctr Reprod Med, Dept Urol, Cleveland, OH 44106 USA
dc.description.affiliationUniv Fed Sao Paulo, Dept Surg, Div Urol, Human Reprod Sect, Sao Paulo, Brazil
dc.description.affiliationRavenshaw Univ, Dept Zool, Sch Life Sci, Cuttack, Orissa, India
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Surg, Div Urol, Human Reprod Sect, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipAmerican Center for Reproductive Medicine, Cleveland Clinic
dc.format.extent285-296
dc.identifierhttp://dx.doi.org/10.1586/14789450.2016.1151357
dc.identifier.citationExpert Review Of Proteomics. Abingdon, v. 13, n. 3, p. 285-296, 2016.
dc.identifier.doi10.1586/14789450.2016.1151357
dc.identifier.issn1478-9450
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57801
dc.identifier.wosWOS:000371706100005
dc.language.isoeng
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofExpert Review Of Proteomics
dc.rightsAcesso restrito
dc.subjectSpermen
dc.subjectLC-MS/MSen
dc.subjectmale infertilityen
dc.subjectpost-translational modificationsen
dc.subjecthumanen
dc.subjectproteomeen
dc.titleSperm proteomics: potential impact on male infertility treatmenten
dc.typeArtigo
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