Identificação e caracterização de Candida spp. isoladas de morcego da espécie Carollia perspicillata na região amazônica
Data
2024-09-03
Autores
Rosa, Camila Ribeiro 
Orientadores
Pascon, Renata Castiglioni
Tipo
Trabalho de conclusão de curso
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Candida spp. são patógenos oportunistas, naturalmente encontrados na microbiota humana e em animais endotérmicos e por serem oportunistas, causam doenças principalmente em pessoas imunocomprometidas, ou seja, com o sistema imunológico em desequilíbrio. Em pacientes imunocomprometidos, como os portadores do vírus HIV, que desenvolveram AIDS, assim como pessoas saudáveis com quedas esporádicas na imunidade ou quebras de barreiras (translocação, mudança de pH, corte ou queimadura acidental nas mucosas), esses microrganismos provocam a infecção fúngica conhecida como candidíase. Em casos mais graves, Candida spp. pode causar infecção de corrente sanguínea (ICS), denominada candidemia. Os morcegos são animais endotérmicos com grande potencial de resistência aos microrganismos; devido a sua capacidade de voar longas distâncias são fontes importantes de dispersão de contaminação ambiental. A espécie Carollia perspicillata, possui em sua microbiota as principais Candida spp. causadoras de candidíase no Brasil e no mundo. O objetivo deste trabalho foi caracterizar cinco isolados de Candida spp. coletados da microbiota de morcego da espécie C. perspicillata, do município de Ouro Preto do Oeste, em Rondônia. Foram empregadas técnicas de microscopia óptica, cultivo em meio diferencial e identificação molecular por sequenciamento do DNA ribossômico pela técnica de Sanger, para identificação dos isolados no nível de espécie; além disso, foram feitos testes de suscetibilidade ao fluconazol e anfotericina B. Com este Trabalho de Conclusão de Curso foi possível fazer uma caracterização macro e micromorfológica destas leveduras, bem como, foi possível confirmar a identificação inicial feita por MALDI-TOF MS e definir a espécie dos isolados como: C. tropicalis, C. krusei, C. parapsilosis, C. orthopsilosis e a quinta definida e confirmada como C. glabrata.
Candida spp. are opportunistic pathogens naturally found in the human microbiota and in endothermic animals. Because they are opportunistic, they cause diseases mainly in immunocompromised individuals, i.e., those with an unbalanced immune system. In immunocompromised patients, such as HIV carriers who have developed AIDS, as well as healthy individuals with sporadic drops in immunity or barrier breakdowns (translocation, pH changes, accidental cuts or burns to the mucous membranes), these microorganisms cause the fungal infection known as candidiasis. In more severe cases, Candida spp. can cause bloodstream infection (BSI), called candidemia. Bats are endothermic animals with a high potential for resistance to microorganisms; due to their ability to fly long distances, they are important sources of environmental contamination. The species Carollia perspicillata has in its microbiota the main Candida spp. that causes candidiasis in Brazil and worldwide. The objective of this study was to characterize five Candida spp. isolates collected from the microbiota of the bat species C. perspicillata, from the municipality of Ouro Preto do Oeste, in Rondônia. Optical microscopy, cultivation in differential medium and molecular identification by sequencing of ribosomal DNA using the Sanger technique were used to identify the isolates at the species level; in addition, susceptibility tests to fluconazole and amphotericin B were performed. With this Final Course Work, it was possible to perform a macro and micromorphological characterization of these yeasts, as well as confirm the initial identification made by MALDI-TOF MS and define the species of the isolates as: C. tropicalis, C. krusei, C. parapsilosis, C. orthopsilosis and the fifth defined and confirmed as C. glabrata.
Candida spp. are opportunistic pathogens naturally found in the human microbiota and in endothermic animals. Because they are opportunistic, they cause diseases mainly in immunocompromised individuals, i.e., those with an unbalanced immune system. In immunocompromised patients, such as HIV carriers who have developed AIDS, as well as healthy individuals with sporadic drops in immunity or barrier breakdowns (translocation, pH changes, accidental cuts or burns to the mucous membranes), these microorganisms cause the fungal infection known as candidiasis. In more severe cases, Candida spp. can cause bloodstream infection (BSI), called candidemia. Bats are endothermic animals with a high potential for resistance to microorganisms; due to their ability to fly long distances, they are important sources of environmental contamination. The species Carollia perspicillata has in its microbiota the main Candida spp. that causes candidiasis in Brazil and worldwide. The objective of this study was to characterize five Candida spp. isolates collected from the microbiota of the bat species C. perspicillata, from the municipality of Ouro Preto do Oeste, in Rondônia. Optical microscopy, cultivation in differential medium and molecular identification by sequencing of ribosomal DNA using the Sanger technique were used to identify the isolates at the species level; in addition, susceptibility tests to fluconazole and amphotericin B were performed. With this Final Course Work, it was possible to perform a macro and micromorphological characterization of these yeasts, as well as confirm the initial identification made by MALDI-TOF MS and define the species of the isolates as: C. tropicalis, C. krusei, C. parapsilosis, C. orthopsilosis and the fifth defined and confirmed as C. glabrata.