Phenotypic and genetic features of Escherichia coli strains showing simultaneous expression of localized and diffuse adherence

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dc.contributor.authorScaletsky, Isabel Cristina Affonso [UNIFESP]
dc.contributor.authorPedroso, Margareth Zabeu [UNIFESP]
dc.contributor.authorSilva, Rosa Maria [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:30:46Z
dc.date.available2016-01-24T12:30:46Z
dc.date.issued1999-03-01
dc.description.abstractWe have previously shown that some Escherichia coli strains isolated from children with diarrhea present the so-called 'localized and diffused adherence (LA/DA) pattern' in which both localized adherence (LA) and diffused adherence (DA) are expressed simultaneously. in the present study, we show that the LA adherence of these strains is genetically and phenotypically similar to that so far described for enteropathogenic E. coli (EPEC) as determined by DNA hybridization and electron microscopy. On the other hand, the DA is encoded by genes not homologous to the DAEC or AIDA-I DNA probes. in addition, the LA/DA strains are able to invade eukaryotic cells both in vitro and in vivo. in the rabbit ileal loop assay their invasion capacity goes beyond the enterocyte and reaches the muscularis mucosae as determined by transmission electron microscopy. These findings suggest that the LA/DA adherence pattern may be linked to a new E. coli virulence category which in the case of the strains studied may be associated to other virulence traits that enable them to more deeply invade the intestinal mucosa. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Div Pediat Gastroenterol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Pediat Gastroenterol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent181-188
dc.identifierhttp://dx.doi.org/10.1111/j.1574-695X.1999.tb01237.x
dc.identifier.citationFems Immunology and Medical Microbiology. Amsterdam: Elsevier B.V., v. 23, n. 3, p. 181-188, 1999.
dc.identifier.doi10.1111/j.1574-695X.1999.tb01237.x
dc.identifier.issn0928-8244
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26038
dc.identifier.wosWOS:000079276600001
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofFems Immunology and Medical Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectdiarrheaen
dc.subjectEscherichia colien
dc.subjectadhesionen
dc.subjectlocalized adherenceen
dc.subjectdiffuse adherenceen
dc.subjectinvasionen
dc.titlePhenotypic and genetic features of Escherichia coli strains showing simultaneous expression of localized and diffuse adherenceen
dc.typeinfo:eu-repo/semantics/article
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