Angiotensin II chronic infusion induces B1 receptor expression in aorta of rats

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dc.contributor.authorCeravolo, Graziela Scalanti
dc.contributor.authorFernandes, Liliam [UNIFESP]
dc.contributor.authorMunhoz, Carolina Demarchi
dc.contributor.authorFernandes, Denise de Castro
dc.contributor.authorTostes, Rita de Cassia Aleixo
dc.contributor.authorLaurindo, Francisco Rafael Martins
dc.contributor.authorScavone, Cristoforo
dc.contributor.authorFortes, Zuleica Bruno
dc.contributor.authorCarvalho, Maria Helena Catelli de
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:49:06Z
dc.date.available2016-01-24T13:49:06Z
dc.date.issued2007-10-01
dc.description.abstractWe investigated whether angiotensin II infusion modulates in vivo the kinin B1 receptor expression and the mechanisms involved in this process. We also evaluated the role of the B1 receptor activation in aorta. Wistar rats received 400 ng/kg per minute of angiotensin II or saline (control rats) infusion during 14 days through an osmotic minipump. To investigate the role of superoxide anion in B1 receptor expression, rats received a reduced nicotinamide-adenine dinucleotide phosphate oxidase inhibitor in the drinking water during 14 days (60 mg/L of apocynin) simultaneously with angiotensin II infusion. Angiotensin II induced B1 receptor expression in the aorta and increased significantly systolic blood pressure, superoxide anion, and the nuclear factor kappa B activity. Apocynin treatment did not alter the blood pressure levels of angiotensin II rats and reduced the B1 receptor expression, superoxide anion generation, and nuclear factor kappa B activity to similar levels of the control rats. Vascular reactivity studies in isolated aorta reveal that B1 receptor agonist promoted endothelium-dependent dilation and increased the NO generation in aorta of angiotensin II rats. NO synthase inhibitor and B1 receptor antagonist inhibited the vasodilation and NO generation, which were not affected by B2 receptor antagonist or indomethacin. These results provide evidence that angiotensin II induces B1 receptor expression in aorta by superoxide anion generation, via reduced nicotinamide-adenine dinucleotide phosphate oxidase, concomitant to nuclear factor kappa B activation. We have also shown that B1 receptor agonist causes endothelium-dependent vasodilation through NO production in aortic rings, suggesting that the B1 receptor expression could be related with the vascular tonus control of angiotensin II rats.en
dc.description.affiliationUniv São Paulo, Dept Pharmacol, Lab Hypertens, Inst Biomed Sci, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Med, Inst Heart, Vasc Biol Lab, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biol Sci, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biol Sci, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.format.extent756-761
dc.identifierhttps://dx.doi.org/10.1161/HYPERTENSIONAHA.107.094706
dc.identifier.citationHypertension. Philadelphia: Lippincott Williams & Wilkins, v. 50, n. 4, p. 756-761, 2007.
dc.identifier.doi10.1161/HYPERTENSIONAHA.107.094706
dc.identifier.issn0194-911X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/30060
dc.identifier.wosWOS:000249586900028
dc.language.isoeng
dc.publisherLippincott Williams & Wilkins
dc.relation.ispartofHypertension
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAngiotensin IIen
dc.subjectB1 receptoren
dc.subjectHypertensionen
dc.subjectSuperoxide anionen
dc.subjectNF-kappa Ben
dc.titleAngiotensin II chronic infusion induces B1 receptor expression in aorta of ratsen
dc.typeinfo:eu-repo/semantics/article
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