Effect of invertebrate serine proteinase inhibitors on carrageenan-induced pleural exudation and bradykinin release

Página do item simplificado
dc.contributor.authorMalavazi-Piza, K. C.
dc.contributor.authorAraujo, M. S.
dc.contributor.authorGodinho, R. O.
dc.contributor.authorTanaka, A. S.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:37:25Z
dc.date.available2016-01-24T12:37:25Z
dc.date.issued2004-10-01
dc.description.abstractThe carrageenan model of pleurisy is described as temporal plasma exudation (1-5 h) with extensive neutrophil infiltration and release of proteinases into the pleural cavity. the aim of this work was to study the effects of serine proteinase inhibitors on the inflammatory process induced by administration of caffageenan to the rat pleural cavity and on release of kinins in pleural exudate. Pleurisy was induced by injecting carrageenan and serine proteinase inhibitors simultaneously into the pleural cavity. the proteinase inhibitors used were: aprotinin, a plasma kallikrein inhibitor; recombinant leech derived tryptase inhibitor-2PL (LDTI-2PL), a plasmin inhibitor; Boophilus microplus trypsin inhibitors (BmTIs); trypsin; plasma kallikrein; plasmin and neutrophil elastase inhibitors; and a synthetic neutrophil elastase inhibitor (EIsynt). Administration of carrageenan with LDTI-2PL and BmTIs induced a marked increase in exudation (143% and 201%) and leukocyte migration (288% and 408%), respectively, when compared to the control group. Pleural exudate from LDTI-2PL and BmTIs plus carrageenan-treated rats showed a significant increase in plasma kallikrein-like activity, measured by chromogenic substrate hydrolysis. the specific inhibition of enzymatic activity with aprotinin confirmed that 50% of S2302 hydrolysis was produced by plasma kallikrein-like enzymes. Kinin release was increased by 97% and 103% in exudates from LDTI-2PL and BmTIs plus carrageenan-treated rats, respectively. Considering that the plasmin inhibitors LDTI-2PL and BmTIs increased exudation, leukocyte migration and bradykinin release, our results suggest an anti-inflammatory role for plasmin in the pleurisy model. (C) 2004 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Farmacol, BR-04044020 São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Farmacol, BR-04044020 São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent1401-1408
dc.identifierhttp://dx.doi.org/10.1016/j.intimp.2004.06.004
dc.identifier.citationInternational Immunopharmacology. Amsterdam: Elsevier B.V., v. 4, n. 10-11, p. 1401-1408, 2004.
dc.identifier.doi10.1016/j.intimp.2004.06.004
dc.identifier.issn1567-5769
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27963
dc.identifier.wosWOS:000223678300012
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofInternational Immunopharmacology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectplasminen
dc.subjectplasma kallikreinen
dc.subjectneutrophil elastaseen
dc.subjectbradykininen
dc.subjectpleurisyen
dc.titleEffect of invertebrate serine proteinase inhibitors on carrageenan-induced pleural exudation and bradykinin releaseen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos