Infection by Trypanosoma cruzi - Identification of a parasite ligand and its host cell receptor

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dc.contributor.authorMagdesian, M. H.
dc.contributor.authorGiordano, R.
dc.contributor.authorUlrich, Alexander Henning [UNIFESP]
dc.contributor.authorJuliano, Maria Aparecida [UNIFESP]
dc.contributor.authorJuliano, Luiz [UNIFESP]
dc.contributor.authorSchumacher, R. I.
dc.contributor.authorColli, W.
dc.contributor.authorAlves, MJM
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:31:24Z
dc.date.available2016-01-24T12:31:24Z
dc.date.issued2001-06-01
dc.description.abstractThe infective trypomastigote stage of Trypanosoma cruzi expresses a set of surface glycoproteins that are known collectively as Tc85 and belong to the gp85/transsialidase supergene family. A member of this family, Tc85-11, with adhesive properties to laminin and cell surfaces was recently cloned. in this report, the Tc85-11 domain for cell binding and its corresponding receptor on epithelial cell LLC-MK2 are described. Using synthetic peptides corresponding to the Tc85-11 carboxyl-terminal segment, we show that the mammalian cell-binding domain colocalizes to the most conserved motif of the trypanosome gp85/trans-sialidase supergene family (VTVXNVFLYNR), Even though Tc85-11 binds to laminin, the 19-residue cell-binding peptide (peptide J) does not contain the laminin-binding site, because it does not bind to laminin or inhibit cell binding to this glycoprotein, the host cell receptor for the peptide was characterized as cytokeratin 18, Addition of anti-cytokeratin antibodies to the culture medium significantly inhibited the infection of epithelial cells by T. cruzi. Tc85-11 is a multiadhesive glycoprotein, encoding at least two different binding sites, one for laminin and one for cytokeratin 18, that allow the parasite to overcome the barriers imposed by cell membranes, extracellular matrices, and basal laminae to reach the definitive host cell. This is the first description of a direct interaction between cytokeratin and a protozoan parasite.en
dc.description.affiliationUniv São Paulo, Inst Quim, Dept Bioquim, BR-05513970 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, EPM, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent19382-19389
dc.identifierhttp://dx.doi.org/10.1074/jbc.M011474200
dc.identifier.citationJournal of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 276, n. 22, p. 19382-19389, 2001.
dc.identifier.doi10.1074/jbc.M011474200
dc.identifier.issn0021-9258
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26560
dc.identifier.wosWOS:000169091000099
dc.language.isoeng
dc.publisherAmer Soc Biochemistry Molecular Biology Inc
dc.relation.ispartofJournal of Biological Chemistry
dc.rightsAcesso aberto
dc.titleInfection by Trypanosoma cruzi - Identification of a parasite ligand and its host cell receptoren
dc.typeArtigo
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