Characterization of a tissue kallikrein inhibitor isolated from Bauhinia bauhinioides seeds: inhibition of the hydrolysis of kininogen related substrates

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dc.contributor.authorOliva, MLV
dc.contributor.authorMendes, C. R.
dc.contributor.authorJuliano, M. A.
dc.contributor.authorChagas, JR
dc.contributor.authorRosa, J. C.
dc.contributor.authorGreene, L. J.
dc.contributor.authorSampaio, M. U.
dc.contributor.authorSampaio, CAM
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T12:30:57Z
dc.date.available2016-01-24T12:30:57Z
dc.date.issued1999-12-01
dc.description.abstractTrypsin inhibitors were purified from a saline extract of Bauhinia bauhinioides seeds by ion-exchange column chromatography on DEAE-Sephadex, gel filtration on Superose 12 column, Mono Q ion-exchange chromatography or, alternatively, by affinity chromatography on trypsin-Sepharose. Both B. bauhinioides isolated inhibitors, BbTI-I and BbTI-II, inhibit trypsin being the dissociation constant 0.6 and 0.36 nM, respectively. BbTI-II only inhibits porcine pancreatic kallikrein hydrolysis of H-Pro-Phe-Arg-AMC (K-i 2.0 nM); the bradykinin-containing sequence LGMISLMKRPPGFSPFRSSRI-NH2 and the two kininogen related flanking quenched substrates Abz-MISLMKRP-EDDnp (K-i 2.0 nM) and Abz-FRSSRQ-EDDnp (K-i 2.5 nM). (C) 1999 Published by Elsevier Science B.V. All rights reserved.en
dc.description.affiliationUNIFESP, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUNIFESP, Escola Paulista Med, Dept Biofis, BR-04044020 São Paulo, Brazil
dc.description.affiliationUSP, Fac Med Ribeirao Preto, Ctr Quim Prot, Ribeirao Preto, Brazil
dc.description.affiliationUnifespUNIFESP, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUNIFESP, Escola Paulista Med, Dept Biofis, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent163-169
dc.identifierhttp://dx.doi.org/10.1016/S0162-3109(99)00075-2
dc.identifier.citationImmunopharmacology. Amsterdam: Elsevier B.V., v. 45, n. 1-3, p. 163-169, 1999.
dc.identifier.doi10.1016/S0162-3109(99)00075-2
dc.identifier.issn0162-3109
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26187
dc.identifier.wosWOS:000084080100027
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofImmunopharmacology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectkallikreinen
dc.subjectserine proteinase inhibitoren
dc.subjectblood clotting enzymesen
dc.subjecttissue kallikrein inhibitoren
dc.titleCharacterization of a tissue kallikrein inhibitor isolated from Bauhinia bauhinioides seeds: inhibition of the hydrolysis of kininogen related substratesen
dc.typeinfo:eu-repo/semantics/article
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