A manual method to obtain platelet rich plasma

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dc.contributor.authorMarques, Fabiana Paulino
dc.contributor.authorIngham, Sheila Jean McNeill [UNIFESP]
dc.contributor.authorForgas, Andrea
dc.contributor.authorFranciozi, Carlos Eduardo da Silveira [UNIFESP]
dc.contributor.authorSasaki, Pedro Henrique
dc.contributor.authorAbdalla, Rene Jorge [UNIFESP]
dc.contributor.institutionHospital do Coração Knee Institute
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:46:51Z
dc.date.available2015-06-14T13:46:51Z
dc.date.issued2014-01-01
dc.description.abstractOBJECTIVE:This study is to report a manual method to obtain platelet rich plasma (PRP).METHODS:For this study 61 ml of peripheral blood was obtained and submitted to centrifugation at 541g for 5 min. The centrifugation separates the blood into three components: red blood cells, buffy coat and platelet rich plasma. Blood and platelet rich plasma samples were sent to the Hospital's Laboratory and platelets and leukocytes were measured.RESULTS:A sample of 637 blood donors was evaluated. The platelet yield efficiency was 86.77% and the increase in platelet concentration factor was 2.89 times. The increase in leukocyte concentration factor was 1.97 times.CONCLUSION:The method described here produces leukocyte-rich and platelet-rich plasma with a high platelet and leukocyte increased factor.Level of Evidence IV, Controlled Laboratory Study.en
dc.description.affiliationHospital do Coração Knee Institute
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Department of Orthopedics and Traumatology
dc.description.affiliationUnifespUNIFESP, EPM, Department of Orthopedics and Traumatology
dc.description.sourceSciELO
dc.format.extent75-77
dc.identifierhttp://dx.doi.org/10.1590/1413-78522014220200798
dc.identifier.citationActa Ortopédica Brasileira. Sociedade Brasileira de Ortopedia e Traumatologia, v. 22, n. 2, p. 75-77, 2014.
dc.identifier.doi10.1590/1413-78522014220200798
dc.identifier.fileS1413-78522014000200075.pdf
dc.identifier.issn1413-7852
dc.identifier.scieloS1413-78522014000200075
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/8166
dc.language.isoeng
dc.publisherSociedade Brasileira de Ortopedia e Traumatologia
dc.relation.ispartofActa Ortopédica Brasileira
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectPlatelet-rich plasmaen
dc.subjectBlood plateletsen
dc.subjectMethods.en
dc.titleA manual method to obtain platelet rich plasmaen
dc.typeinfo:eu-repo/semantics/article
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