Virulence properties of Escherichia coli O111:H12 strains

dc.contributor.authorMonteiro-Neto, Valério
dc.contributor.authorCampos, Leila Carvalho
dc.contributor.authorFerreira, Antonio José Piantino
dc.contributor.authorGomes, Tania Aparecida Tardelli [UNIFESP]
dc.contributor.authorTrabulsi, Luiz Rachid
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.abstractSeventeen Escherichia coli O111:H12 strains isolated from the feces of children with acute diarrhea were studied with regard to their adherence properties and other virulence characteristics. All strains showed an aggregative adherence pattern to HEp-2 cells and agglutinated bovine and sheep red cells in the presence of mannose. These strains did not have gene sequences homologous to the aggregative adherence fimbria I gene and did not react with any of the DNA probes used to detect other virulence genes in enteropathogens. With one exception, the O111:H12 strains did not induce fluid accumulation in the rabbit ileal loop assay, although 16 of the strains had the enteroaggregative E. coli heat-stable enterotoxin 1 (EAST1) gene sequences. A 60-70 MDa plasmid was present in 16 of the strains studied. We conclude that the O111 :H12 serotype, one of the first E. coli identified in infantile diarrhea, belongs to the enteroaggregative E. coli category but the genes encoding its adherence phenotype are distinct from those previously described.en
dc.description.affiliationUNIV São Paulo, INST CIENCIAS BIOMED, DEPT MICROBIOL, BR-05508900 São Paulo, BRAZIL
dc.description.affiliationUNIV São Paulo, FAC MED VET & ZOOTECNIA, DEPT PATOL, BR-05340900 São Paulo, BRAZIL
dc.description.affiliationUniversidade Federal de São Paulo, ESCOLA PAULISTA MED, DEPT MICROBIOL IMMUNOL & PARASITOL, BR-04023062 São Paulo, BRAZIL
dc.description.affiliationUnifespUniversidade Federal de São Paulo, ESCOLA PAULISTA MED, DEPT MICROBIOL IMMUNOL & PARASITOL, BR-04023062 São Paulo, BRAZIL
dc.description.sourceWeb of Science
dc.identifier.citationFems Microbiology Letters. Hoboken: Wiley-Blackwell, v. 146, n. 1, p. 123-128, 1997.
dc.relation.ispartofFems Microbiology Letters
dc.rightsAcesso aberto
dc.subjectenteroaggregative Escherichia colien
dc.subjectadherence patternen
dc.subjectvirulence propertiesen
dc.subjectmannose-resistant hemagglutination activityen
dc.titleVirulence properties of Escherichia coli O111:H12 strainsen