Production and release of heat-labile toxin by wild-type human-derived enterotoxigenic Escherichia coli

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dc.contributor.authorSimoes Lasaro, Melissa Ang
dc.contributor.authorRodrigues, Juliana Falcao
dc.contributor.authorMathias-Santos, Camila
dc.contributor.authorGuth, Beatriz Ernestina Cabilio [UNIFESP]
dc.contributor.authorRegua-Mangia, Adriana
dc.contributor.authorPiantino Ferreira, Antonio Jose
dc.contributor.authorTakagi, Mickie
dc.contributor.authorCabrera-Crespo, Joaquin
dc.contributor.authorSbrogio-Almeida, Maria Elisabete
dc.contributor.authorSouza Ferreira, Luis Carlos de
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFdn Oswaldo Cruz
dc.contributor.institutionInst Butantan
dc.date.accessioned2016-01-24T12:41:29Z
dc.date.available2016-01-24T12:41:29Z
dc.date.issued2006-10-01
dc.description.abstractProduction and release of heat-labile toxin (LT) by wild-type enterotoxigenic Escherichia coli (ETEC) strains, isolated from diarrheic and asymptomatic Brazilian children, was studied under in vitro and in vivo conditions. Based on a set of 26 genetically diverse LT+ enterotoxigenic E. coli strains, cell-bound LT concentrations varied from 49.8 to 2415 ng mL(-1). the amounts of toxin released in culture supernatants ranged from 0% to 50% of the total synthesized toxin. the amount of LT associated with secreted membrane vesicles represented < 5% of the total toxin detected in culture supernatants. ETEC strains secreting higher amounts of LT, but not those producing high intracellular levels of cell-bound toxin, elicited enhanced fluid accumulation in tied rabbit ileal loops, suggesting that the strain-specific differences in production and secretion of LT correlates with symptoms induced in vivo. However, no clear correlation was established between the ability to produce and secrete LT and the clinical symptoms of the infected individuals. the present results indicate that production and release of LT by wild-type human-derived ETEC strains are heterogeneous traits under both in vitro and in vivo growth conditions and may impact the clinical outcomes of infected individuals.en
dc.description.affiliationUniv São Paulo, Dept Microbiol, Inst Ciencias Biomed, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.affiliationFdn Oswaldo Cruz, Escola Nacl Saude Publ, Rio de Janeiro, Brazil
dc.description.affiliationUniv São Paulo, Dept Patol, Fac Med Vet & Zootecn, São Paulo, Brazil
dc.description.affiliationInst Butantan, Div Desenvolvimento Tecnol & Prod, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent123-131
dc.identifierhttp://dx.doi.org/10.1111/j.1574-695X.2006.00134.x
dc.identifier.citationFems Immunology and Medical Microbiology. Oxford: Blackwell Publishing, v. 48, n. 1, p. 123-131, 2006.
dc.identifier.doi10.1111/j.1574-695X.2006.00134.x
dc.identifier.issn0928-8244
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/29166
dc.identifier.wosWOS:000240402700016
dc.language.isoeng
dc.publisherBlackwell Publishing
dc.relation.ispartofFems Immunology and Medical Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectheat-labile toxinen
dc.subjectenterotoxigenic Escherichia colien
dc.subjectlabile toxin production/secretionen
dc.subjectdiarrheal diseaseen
dc.titleProduction and release of heat-labile toxin by wild-type human-derived enterotoxigenic Escherichia colien
dc.typeinfo:eu-repo/semantics/article
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