Enhanced production of specific IgG4, IgE, IgA and TGF-beta in sera from patients with the juvenile form of paracoccidioidomycosis

dc.contributor.authorMamoni, R. L.
dc.contributor.authorNouer, S. A.
dc.contributor.authorOliveira, S. J.
dc.contributor.authorMusatti, Chloe Camba [UNIFESP]
dc.contributor.authorRossi, C. L.
dc.contributor.authorCamargo, Zoilo Pires [UNIFESP]
dc.contributor.authorBlotta, MHSL
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.abstractParacoccidioidomycosis (PCM) occurs in two distinct forms, the acute or juvenile form (H), and the chronic or adult form (AT). To clarify the basis of this dichotomy, specific IgG subclasses, IgA and IgE anti-gp43 were measured by enzyme-linked immunosorbent assay, in patients with different forms of PCM. Serum levels of tumor necrosis factor-alpha, interleukin (IL)-6, IL-8, macrophage inflammatory protein (MIP)-1alpha and transforming growth factor (TGF)-beta were also quantified. We show here that JF patients have significantly higher titers of IgE antibodies against gp43, an immunodominant antigen specific for Paracoccidioides brasiliensis, than do patients with the unifocal adult form (UF-AF, isolated lesions). Patients with the multifocal adult form (MF-AF, lesions in more than one organ) also produced elevated levels of anti-P. brasiliensis IgE. Furthermore, specific IgE levels were correlated with IgG4, IgA and eosinophilia. Patients with JF showed eosinophilia and increased levels of TGF-beta, a switching factor for IgA. These results indicate a T helper (Th)-2 pattern of cytokine expression in both the JF and the MF-AF of PCM. On the other hand, patients with UF-AF had a significantly lower production of specific IgE, IgG4 and IgA than was seen in the other patient groups.en
dc.description.affiliationUniv Estadual Campinas, Fac Med Sci, Dept Clin Pathol, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Clementino Fraga Filho Univ Hosp, Rio de Janeiro, Brazil
dc.description.affiliationUniv Fed Uberlandia, Dept Pathol, BR-38400 Uberlandia, MG, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Discipline Cellular Biol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Discipline Cellular Biol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.identifier.citationMedical Mycology. Oxford: B I O S Scientific Publishers Ltd, v. 40, n. 2, p. 153-159, 2002.
dc.publisherB I O S Scientific Publishers Ltd
dc.relation.ispartofMedical Mycology
dc.rightsAcesso restrito
dc.subjectIgG subclassesen
dc.titleEnhanced production of specific IgG4, IgE, IgA and TGF-beta in sera from patients with the juvenile form of paracoccidioidomycosisen