Molecular cloning, sequence analysis and expression of the snake follicle-stimulating hormone receptor

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dc.contributor.authorBluhm, Ana Paula Canel
dc.contributor.authorToledo, Rodrigo de Almeida
dc.contributor.authorMesquita, Fernando Manetti
dc.contributor.authorPimenta, Maristela Taliari [UNIFESP]
dc.contributor.authorFernandes, Flora Maria de Campos
dc.contributor.authorRibela, Maria Teresa Carvalho Pinto
dc.contributor.authorLazari, Maria de Fatima Magalhaes [UNIFESP]
dc.contributor.institutionButantan Inst
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:37:14Z
dc.date.available2016-01-24T12:37:14Z
dc.date.issued2004-07-01
dc.description.abstractFollicle-stimulating hormone (FSH) and luteinizing hormone (LH) control gonadal function in mammalian and many non-mammalian vertebrates through the interaction with their receptors, FSHR and LHR. Although the same is true for some reptilian species, in Squamata (lizards and snakes) there is no definitive evidence for the presence of either two distinct gonadotropins or two distinct gonadotropin receptors. Our aim was to characterize the gonadotropin receptor(s) of the Bothrops jararaca snake. Using a cDNA library from snake testis and amplification of the 5'-cDNA ending, we cloned a cDNA related to FSHR. Attempts to clone a cDNA more closely related to LHR were unsuccessful. Expression of FSHR mRNA was restricted to gonadal tissues. the snake FSHR is a G protein-coupled receptor with 673 amino acids, and the aminoterminal domain with 346 amino acids consists of a nine leucine-rich repeat-containing subdomain (LRR) flanked by two cysteine-rich subdomains. the beta-strands in the LRR are conserved with exception of the third, a region that may be important for FSH binding. in contrast with mammalian, avian and amphibian FSHRs, the snake FSHR presents amino acid deletions in the carboxyterminal region of the extracellular domain which are also seen in fish and lizard FSHRs. cAMP assays with the recombinant protein transiently expressed in HEK-293 cells showed that the snake FSHR is more sensitive to human FSH (hFSH) than to human chorionic gonadotropin. Phylogenetic analysis indicated that the squamate FSHRs group separately from mammalian FSHRs. Our data are consistent with the apparently unique gonadotropin-receptor system in Squamata reptilian subgroup. Knowledge about the snake FSHR structure may help identify structural determinants for receptor function. (C) 2004 Elsevier Inc. All rights reserved.en
dc.description.affiliationButantan Inst, Dept Pharmacol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Museum Zool, Mol Biol Sect, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, IPEN, CNEN, Bioengn Dept,Nucl Energy Inst, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Pharmacol, Expt Endocrinol Unit, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Pharmacol, Expt Endocrinol Unit, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipIDFAPESP: 99/00342-0pt
dc.description.sponsorshipIDFAPESP: 02/08986-8pt
dc.description.sponsorshipIDFAPESP: 01/01793-7pt
dc.description.sponsorshipIDFAPESP: 02/03818-0pt
dc.description.sponsorshipIDFAPESP: 02/12379-0pt
dc.format.extent300-311
dc.identifierhttps://dx.doi.org/10.1016/j.ygcen.2004.03.014
dc.identifier.citationGeneral and Comparative Endocrinology. San Diego: Academic Press Inc Elsevier Science, v. 137, n. 3, p. 300-311, 2004.
dc.identifier.doi10.1016/j.ygcen.2004.03.014
dc.identifier.issn0016-6480
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/27817
dc.identifier.wosWOS:000222341100009
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofGeneral and Comparative Endocrinology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectGonadotropin receptorsen
dc.subjectFSH and LH receptoren
dc.subjectCloningen
dc.subjectSnakeen
dc.subjectBothrops jararacaen
dc.titleMolecular cloning, sequence analysis and expression of the snake follicle-stimulating hormone receptoren
dc.typeinfo:eu-repo/semantics/article
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