In vitro susceptibility of antifungal drugs against Sporothrix brasiliensis recovered from cats with sporotrichosis in Brazil

dc.contributor.authorNogueira Brilhante, Raimunda Samia [UNIFESP]
dc.contributor.authorRodrigues, Anderson Messias [UNIFESP]
dc.contributor.authorCosta Sidrim, Jose Julio [UNIFESP]
dc.contributor.authorGadelha Rocha, Marcos Fabio [UNIFESP]
dc.contributor.authorPereira, Sandro Antonio
dc.contributor.authorFerreira Gremiao, Isabella Dib
dc.contributor.authorPacheco Schubach, Tania Maria
dc.contributor.authorde Camargo, Zoilo Pires [UNIFESP]
dc.description.abstractSporotrichosis is an important subcutaneous mycosis of humans and animals. Classically, the disease is acquired upon traumatic inoculation of Sporothrix propagules from contaminated soil and plant debris. In addition, the direct horizontal transmission of Sporothrix among animals and the resulting zoonotic infection in humans highlight an alternative and efficient rout of transmission through biting and scratching. Sporothrix brasiliensis is themost virulent species of the Sporothrix schenckii complex and is responsible for the long-lasting outbreak of feline sporotrichosis in Brazil. However, antifungal susceptibility data of animal-borne isolates is scarce. Therefore, this study evaluated the in vitro activity of amphotericin B, caspofungin, itraconazole, voriconazole, fluconazole, and ketoconazole against animal-borne isolates of S. brasiliensis. The susceptibility tests were performed through broth microdilution (M38-A2). The results show the relevant activity of itraconazole, amphotericin B, and ketoconazole against S. brasiliensis, with the following MIC ranges: 0.125-2, 0.125-4 and 0.0312-2 mu g/ml, respectively. Caspofungin was moderately effective, displaying higher variation in MIC values (0.25-64 mu g/ml). Voriconazole (2-64 mu g/ml) and fluconazole (62.5-500 mu g/ml) showed low activity against S. brasiliensis strains. This study contributed to the characterization of the in vitro antifungal susceptibility of strains of S. brasiliensis recovered from cats with sporotrichosis, which have recently been considered the main source of human infections.en
dc.description.affiliationUniv Fed Ceara, Specialized Med Mycol Ctr, Postgrad Program Med Microbiol, Fortaleza, Ceara, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Cellular Biol Div, Sao Paulo, Brazil
dc.description.affiliationFundacao Oswaldo Cruz, Evandro Chagas Natl Inst Infect Dis, Rio De Janeiro, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Cellular Biol Div, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipSao Paulo Research Foundation (FAPESP)
dc.description.sponsorshipNational Council for Scientific and Technological Development (CNPq 304779/20113)
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.description.sponsorshipPrograma Estrategico de Apoio a Pesquisa em Saude VI - Fiocruz/CNPq
dc.description.sponsorshipFundacao Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ)
dc.description.sponsorshipIDFAPESP: 2011/07350-1
dc.description.sponsorshipIDFAPESP: 2009/54024-2
dc.description.sponsorshipIDCNPq: 304779/20113
dc.description.sponsorshipIDCAPES: AE1-0052-000650100/11
dc.description.sponsorshipIDFiocruz/CNPq: 407771/2013-3
dc.description.sponsorshipIDFAPERJ: E-26/102.255/2013
dc.identifier.citationMedical Mycology. Oxford, v. 54, n. 3, p. 275-279, 2016.
dc.publisherOxford Univ Press
dc.relation.ispartofMedical Mycology
dc.rightsAcesso aberto
dc.subjectSporothrix brasiliensisen
dc.subjectantifungal susceptibilityen
dc.subjectSporothrix schenckii complexen
dc.titleIn vitro susceptibility of antifungal drugs against Sporothrix brasiliensis recovered from cats with sporotrichosis in Brazilen