Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp citri during Citrus sinensis infection

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dc.citation.volume17]
dc.contributor.authorMoreira, Leandro M.
dc.contributor.authorSoares, Marcia R.
dc.contributor.authorFacincani, Agda P.
dc.contributor.authorFerreira, Cristiano B.
dc.contributor.authorFerreira, Rafael M.
dc.contributor.authorFerro, Maria I. T.
dc.contributor.authorGozzo, Fabio C.
dc.contributor.authorFelestrino, Erica B.
dc.contributor.authorAssis, Renata A. B.
dc.contributor.authorGarcia, Camila Carriao M.
dc.contributor.authorSetubal, Joao C. [UNIFESP]
dc.contributor.authorFerro, Jesus A.
dc.contributor.authorde Oliveira, Julio C. F. [UNIFESP]
dc.coverageLondon
dc.date.accessioned2020-06-26T16:30:16Z
dc.date.available2020-06-26T16:30:16Z
dc.date.issued2017
dc.description.abstractBackground: Xanthomonas citri subsp. citri (Xac) is the causal agent of citrus canker. A proteomic analysis under in planta infectious and non-infectious conditions was conducted in order to increase our knowledge about the adaptive process of Xac during infection. Results: For that, a 2D-based proteomic analysis of Xac at 1, 3 and 5 days after inoculation, in comparison to Xac growth in NB media was carried out and followed by MALDI-TOF-TOF identification of 124 unique differentially abundant proteins. Among them, 79 correspond to up-regulated proteins in at least one of the three stages of infection. Our results indicate an important role of proteins related to biofilm synthesis, lipopolysaccharides biosynthesis, and iron uptake and metabolism as possible modulators of plant innate immunity, and revealed an intricate network of proteins involved in reactive oxygen species adaptation during Plants'Oxidative Burst response. We also identified proteins previously unknown to be involved in Xac-Citrus interaction, including the hypothetical protein XAC3981. A mutant strain for this gene has proved to be non-pathogenic in respect to classical symptoms of citrus canker induced in compatible plants. Conclusions: This is the first time that a protein repertoire is shown to be active and working in an integrated manner during the infection process in a compatible host, pointing to an elaborate mechanism for adaptation of Xac once inside the plant.en
dc.description.affiliationUniv Fed Ouro Preto, Inst Ciencias Exatas & Biol, Dept Ciencias Biol DECBI, Ouro Preto, MG, Brazil
dc.description.affiliationUniv Fed Ouro Preto, Nucleo Pesquisas Ciencias Biol NUPEB, Ouro Preto, MG, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Inst Quim, Dept Bioquim DBq, Rio De Janeiro, RJ, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Dept Tecnol, Fac Ciencias Agr & Vet Jaboticabal, Jaboticabal, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, UNICAMP, Inst Quim, Campinas, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, Dept Ciencias Biol, Diadema, SP, Brazil
dc.description.affiliationVirginia Tech, Biocomplex Inst, Blacksburg, VA USA
dc.description.affiliationUnifespUniv Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, Dept Ciencias Biol, Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipBIGA grant-CAPES
dc.description.sponsorshipIDCAPES
dc.description.sponsorshipIDFAPESP: 04/02006-7
dc.description.sponsorshipIDFundacao de Amparo a Pesquisa do Estado de Minas Gerais: CBB-APQ-04425-10
dc.description.sponsorshipIDBIGA grant-CAPES: 3385/2013
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1186/s12866-017-1063-x]
dc.identifier.citationBmc Microbiology. London, v. 17, p. -, 2017.
dc.identifier.doi10.1186/s12866-017-1063-x
dc.identifier.fileWOS000405421700003.pdf
dc.identifier.issn1471-2180
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/53453
dc.identifier.wosWOS:000405421700003
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofBmc Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectXanthomonasen
dc.subjectadaptation mechanismsen
dc.subjectBiofilmen
dc.subjectIron uptake and metabolismen
dc.subjectPlants'Oxidative bursten
dc.subjectLPS modulationen
dc.titleProteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp citri during Citrus sinensis infectionen
dc.typeinfo:eu-repo/semantics/article
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