SPM-1-producing Pseudomonas aeruginosa ST277 clone recovered from microbiota of migratory birds

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dc.citation.issue3
dc.citation.volume90
dc.contributor.authorMartins, Willames M. B. S. [UNIFESP]
dc.contributor.authorNarciso, Ana Clara [UNIFESP]
dc.contributor.authorCayo, Rodrigo [UNIFESP]
dc.contributor.authorSantos, Stefanie Vanessa
dc.contributor.authorFehlberg, Lorena C. C. [UNIFESP]
dc.contributor.authorRamos, Patricia Locosque
dc.contributor.authorda Cruz, Joao Batista
dc.contributor.authorGales, Ana Cristina [UNIFESP]
dc.coverageNew York
dc.date.accessioned2020-07-08T13:09:32Z
dc.date.available2020-07-08T13:09:32Z
dc.date.issued2018
dc.description.abstractThe production of Sao Paulo metallo-O-lactamase (SPM-1) is the most common carbapenem resistance mechanism detected among multidrug-resistant Pseudomonas aeruginosa clinical isolates in Brazil. Dissemination of SPM-1-producing P. aeruginosa has been restricted to the nosocomial settings, with sporadic reports of environmental isolates due to contamination by hospital sewage. Herein, we described the detection and molecular characterization of SPM-1-producing P. aeruginosa recovered from the microbiota of migratory birds in Brazil. Three hundred gram-negative bacilli were recovered from cloacal and choanal swabs of Dendrocygna viduata during a surveillance study for detection of carbapenem-resistant isolates. All isolates were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing. MICs were determined by agar dilution, except for polymyxin B. Antibiotic resistance genes were detected by polymerase chain reaction (PCR) followed by DNA sequencing. Transcriptional levels of oprD and efflux system encoding genes were also carried out by quantitative real-time PCR. Nine imipenem-resistant P. aeruginosa isolates were recovered with 7 of them carrying bla(SPM-1). Additional resistance genes (rmtD-1, bla(OXA-56), aacA4, and aac(6')-Ib-cr) were also detected in all 9 isolates. The SPM-1-producing isolates showed high MICs for all beta-lactams, fluoroquinolones, and aminoglycosides, being susceptible only to polymyxin B. Interestingly, all isolates showed the same PFGE pattern and belonged to ST277. Overexpression of MexXY-OprM and MexAB-OprM was observed in those isolates that did not harbor bla(SPM-1). Our results suggest that migratory birds might have played a role in the dissemination of SPM-1-producing P. aeruginosa within the Brazilian territory. (C) 2017 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Internal Med, Div Infect Dis,Lab Alerta, Sao Paulo, Brazil
dc.description.affiliationFundacao Parque Zool Sao Paulo, Dept Pesquisas Aplicadas, Sao Paulo, SP, Brazil
dc.description.affiliationFPZSP, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Internal Med, Div Infect Dis,Lab Alerta, Sao Paulo, Brazil
dc.description.provenanceMade available in DSpace on 2020-07-08T13:09:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2018en
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.description.sponsorshipNational Council for Science and Technological Development
dc.description.sponsorshipIDFAPESP: 2017/02258-6, 2014/12224-3
dc.description.sponsorshipIDCAPES PNPD 20131991
dc.description.sponsorshipIDNational Council for Science and Technological Development: 305535/2014-5
dc.format.extent221-227
dc.identifierhttp://dx.doi.org/10.1016/j.diagmicrobio.2017.11.003
dc.identifier.citationDiagnostic Microbiology And Infectious Disease. New York, v. 90, n. 3, p. 221-227, 2018.
dc.identifier.doi10.1016/j.diagmicrobio.2017.11.003
dc.identifier.fileWOS000426228700013.pdf
dc.identifier.issn0732-8893
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54060
dc.identifier.wosWOS:000426228700013
dc.language.isoeng
dc.publisherElsevier Science Inc
dc.relation.ispartofDiagnostic Microbiology And Infectious Disease
dc.rightsAcesso aberto
dc.subjectAntimicrobial resistanceen
dc.subjectCarbapenemaseen
dc.subjectEnvironmental microbiologyen
dc.subjectDendrocygna viduataen
dc.titleSPM-1-producing Pseudomonas aeruginosa ST277 clone recovered from microbiota of migratory birdsen
dc.typeArtigo
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