ARF6, PI3-kinase and host cell actin cytoskeleton in Toxoplasma gondii cell invasion

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dc.contributor.authorSilva, Claudio Vieira da [UNIFESP]
dc.contributor.authorSilva, Erika Alves da [UNIFESP]
dc.contributor.authorCruz, Mario Costa [UNIFESP]
dc.contributor.authorChavrier, Philippe
dc.contributor.authorMortara, Renato Arruda [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionInst Curie
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.date.accessioned2016-01-24T13:52:11Z
dc.date.available2016-01-24T13:52:11Z
dc.date.issued2009-01-16
dc.description.abstractToxoplasma gondii infects a variety of different cell types in a range of different hosts. Host cell invasion by T. gondii occurs by active penetration of the host cell, a process previously described as independent of host actin polymerization. Also, the parasitophorous vacuole has been shown to resist fusion with endocytic and exocytic pathways of the host cell. ADP-ribosylation factor-6 (ARF6) belongs to the ARE family of small GTP-binding proteins. ARF6 regulates membrane trafficking and actin cytoskeleton rearrangements at the plasma membrane. Here, we have observed that ARF6 is recruited to the parasitophorous vacuole of tachyzoites of T. gondii RH strain and it also plays an important role in the parasite cell invasion with activation of PI3-kinase and recruitment of PIP(2) and PIP(3) to the parasitophorous vacuole of invading parasites. Moreover, it was verified that maintenance of host cell actin cytoskeleton integrity is important to parasite invasion. (C) 2008 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationInst Curie, CNRS, UMR144, F-75248 Paris, France
dc.description.affiliationUniv Fed Uberlandia, Inst Ciencias Biomed, BR-38400902 Uberlandia, MG, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 07/54300-4
dc.format.extent656-661
dc.identifierhttp://dx.doi.org/10.1016/j.bbrc.2008.11.108
dc.identifier.citationBiochemical and Biophysical Research Communications. San Diego: Academic Press Inc Elsevier Science, v. 378, n. 3, p. 656-661, 2009.
dc.identifier.doi10.1016/j.bbrc.2008.11.108
dc.identifier.issn0006-291X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31251
dc.identifier.wosWOS:000262343200064
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBiochemical and Biophysical Research Communications
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectToxoplasma gondiien
dc.subjectHost cell invasionen
dc.subjectParasitophorous vacuoleen
dc.subjectARF6en
dc.subjectPI3-kinaseen
dc.subjectActin cytoskeletonen
dc.titleARF6, PI3-kinase and host cell actin cytoskeleton in Toxoplasma gondii cell invasionen
dc.typeinfo:eu-repo/semantics/article
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