Early Diagnosis of Prostate Cancer by Citrate Determination in Urine with Europium-Oxytetracycline Complex

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dc.contributor.authorSilva, Flavia R. O. [UNIFESP]
dc.contributor.authorNabeshima, Camila Tiemi [UNIFESP]
dc.contributor.authorBellini, Maria H.
dc.contributor.authorCourrol, Lilia C. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionIPEN CNEN SP
dc.date.accessioned2016-01-24T14:27:33Z
dc.date.available2016-01-24T14:27:33Z
dc.date.issued2012-08-01
dc.description.abstractNormal prostate tissue contains high levels of citrate. in the presence of prostate cancer, the citrate level is diminished. in this paper we show that it is possible to use europium oxytetracycline complex as a citrate fluorescent probe and consequently as a prostate cancer probe. We analyzed normal nude male mice urine and urine from nude male mice in which prostate cancer was induced by intraprostatic inoculation of DU145 cells. the urine samples were collected from the animals at the 7th, 14th, 21st, and 35th days after the surgery procedures. the intensity of europium emission at 615 nm in europium oxytetracycline complex in the presence of citrate increases linearly. the citrate concentrations were determined from a calculated calibration curve. A concentration decrease in malignant prostate urine from the normal (PBS group) urine value from similar to 8.0 mM to similar to 2.4 mM (tumor group at 35th day) was found. the obtained results indicated that europium oxytetracycline provides a significant biomarker for prostate cancer detection with a direct, accurate, noninvasive, and non-enzymatic method for measurement of citrate in biological fluids.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Nefrol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Ciencias Exatas & Terra, Diadema, Brazil
dc.description.affiliationIPEN CNEN SP, Inst Pesquisas Energet & Nucl, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Nefrol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Ciencias Exatas & Terra, Diadema, Brazil
dc.description.sourceWeb of Science
dc.format.extent958-961
dc.identifierhttp://dx.doi.org/10.1366/11-06546
dc.identifier.citationApplied Spectroscopy. Frederick: Soc Applied Spectroscopy, v. 66, n. 8, p. 958-961, 2012.
dc.identifier.doi10.1366/11-06546
dc.identifier.issn0003-7028
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35161
dc.identifier.wosWOS:000306720600013
dc.language.isoeng
dc.publisherSoc Applied Spectroscopy
dc.relation.ispartofApplied Spectroscopy
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCitrateen
dc.subjectEuropiumen
dc.subjectTetracyclineen
dc.subjectFluorescenceen
dc.subjectFluorescent probeen
dc.subjectProstate canceren
dc.titleEarly Diagnosis of Prostate Cancer by Citrate Determination in Urine with Europium-Oxytetracycline Complexen
dc.typeinfo:eu-repo/semantics/article
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