Differential expression of toll-like receptor signaling cascades in LPS-tolerant human peripheral blood mononuclear cells

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dc.contributor.authorMendes, Marialice Erdelyi [UNIFESP]
dc.contributor.authorBaggio-Zappia, Giovana Lotici [UNIFESP]
dc.contributor.authorColo Brunialti, Milena Karina [UNIFESP]
dc.contributor.authorFernandes, Maria da Luz [UNIFESP]
dc.contributor.authorRapozo, Marjorie Marini [UNIFESP]
dc.contributor.authorSalomao, Reinaldo [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:06:16Z
dc.date.available2016-01-24T14:06:16Z
dc.date.issued2011-03-01
dc.description.abstractPre-exposure to low doses of LPS induces resistance to a lethal challenge, a phenomenon known as endotoxin tolerance. in this study, tolerance was induced in human PBMC by culturing cells with 1 ng/mL LPS for 48 h. Cells were subsequently challenged with 100 ng/mL LPS for 2, 6 and 24 h, and the expression of 84 genes encoding proteins involved in the TLR signaling pathway was evaluated at each time point by PCR array. LPS pretreatment did not modulate the expression of TLR4 and CD14 on the surface of monocytes. A gene was defined as tolerized when LPS pretreatment reversed the effect of LPS challenge on the expression of the gene or as non-tolerized when LPS pretreatment did not reverse the effects of LPS challenge. We observed impaired signal transduction through the NF-kappa B, JNK, ERK and TRIF pathways, whereas expression of p38 pathway-related genes was preserved in LPS-tolerant cells. These results show a distinct regulation of the TLR pathway cascades during tolerance; this may account for the differential gene expression of some inflammatory mediators, such as up-regulation of IL-10 and COX2 as well as down-regulation of INF-alpha and IL-12. Depending on the effect of LPS-induced gene up-regulation or down-regulation, tolerance, as a reversion of such LPS effects, may result in repression or induction of gene expression. (C) 2010 Elsevier GmbH. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Div Infect Dis, Escola Paulista Med, Dept Med, BR-04039032 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Infect Dis, Escola Paulista Med, Dept Med, BR-04039032 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2006/58744-1
dc.description.sponsorshipIDFAPESP: 2007/56960-7
dc.format.extent285-295
dc.identifierhttp://dx.doi.org/10.1016/j.imbio.2010.07.008
dc.identifier.citationImmunobiology. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 216, n. 3, p. 285-295, 2011.
dc.identifier.doi10.1016/j.imbio.2010.07.008
dc.identifier.issn0171-2985
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/33525
dc.identifier.wosWOS:000288722500002
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofImmunobiology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectGene expressionen
dc.subjectInnate immunityen
dc.subjectLPSen
dc.subjectp38en
dc.subjectPBMCen
dc.subjectToleranceen
dc.subjectToll-like receptorsen
dc.titleDifferential expression of toll-like receptor signaling cascades in LPS-tolerant human peripheral blood mononuclear cellsen
dc.typeinfo:eu-repo/semantics/article
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