Cloning and characterization of a gene encoding a novel immunodominant antigen of Trypanosoma cruzi

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dc.contributor.authorLesenechal, M.
dc.contributor.authorDuret, L.
dc.contributor.authorCano, M. I.
dc.contributor.authorMortara, R. A.
dc.contributor.authorJolivet, M.
dc.contributor.authorCamargo, M. E.
dc.contributor.authordaSilveira, J. F.
dc.contributor.authorParanhosBaccala, G.
dc.contributor.institutionECOLE NORMALE SUPER LYON
dc.contributor.institutionLAB BIOMETRIE
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionBIOLAB MERIEUX
dc.date.accessioned2016-01-24T12:30:24Z
dc.date.available2016-01-24T12:30:24Z
dc.date.issued1997-08-01
dc.description.abstractA Trypanosoma cruzi genomic expression library was screened with a pool of sera obtained from chronic chagasic patients. the recombinant antigen (Tc40) isolated from this library reacted with a large number of serum samples of chronic chagasic patients, suggesting that the presence of anti-Tc40 antibodies may be specifically associated to Chagas' disease. the full-length sequence of the Tc40 gene was determined after isolation of genomic and cDNA clones. the Tc40 cDNA includes a large open reading frame (2745 bp-long) that encodes a polypeptide of 100 kDa without any homology with previously described T. cruzi sequences. in contrast with other T. cruzi antigens whose immunodominant B-cell epitopes are composed by amino acid repetitive motifs, Tc40 does not show any amino acid repetition. Antibodies against the Tc40 recombinant protein reacted with three native polypeptides of 100, 41 and 38 kDa which are lightly associated with membranes or cytoskeleton and expressed in all developmental stages of the parasite life cycle. A transcript of 3.9-kb was detected in Northern blot analysis which is large enough to encode a 100 kDa polypeptide. Tc40 genes were mapped on a chromosomal band of 1.1 Mbp and in a few copies per haploid genome in the G strain. (C) 1997 Elsevier Science B.V.en
dc.description.affiliationECOLE NORMALE SUPER LYON,UNITE MIXTE CNRS BIOMERIEUX,F-69364 LYON 07,FRANCE
dc.description.affiliationLAB BIOMETRIE,CNR,URA 2055,VILLEURBANNE,FRANCE
dc.description.affiliationESCOLA PAULISTA MED,DEPT PARASITOL,BR-04023 São Paulo,BRAZIL
dc.description.affiliationBIOLAB MERIEUX,São Paulo,BRAZIL
dc.description.affiliationUnifespESCOLA PAULISTA MED,DEPT PARASITOL,BR-04023 São Paulo,BRAZIL
dc.description.sourceWeb of Science
dc.format.extent193-204
dc.identifierhttp://dx.doi.org/10.1016/S0166-6851(97)00068-6
dc.identifier.citationMolecular and Biochemical Parasitology. Amsterdam: Elsevier B.V., v. 87, n. 2, p. 193-204, 1997.
dc.identifier.doi10.1016/S0166-6851(97)00068-6
dc.identifier.issn0166-6851
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/25771
dc.identifier.wosWOS:A1997XL35000007
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMolecular and Biochemical Parasitology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectcloningen
dc.subjectimmunodominant antigenen
dc.subjectT-cruzien
dc.titleCloning and characterization of a gene encoding a novel immunodominant antigen of Trypanosoma cruzien
dc.typeinfo:eu-repo/semantics/article
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