Rmcystatin3, a cysteine protease inhibitor from Rhipicephalus microplus hemocytes involved in immune response

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dc.contributor.authorLu, Stephen [UNIFESP]
dc.contributor.authorSoares, Tatiane Sanches [UNIFESP]
dc.contributor.authorVaz Junior, Itabajara S.
dc.contributor.authorLovato, Diogo Ventura [UNIFESP]
dc.contributor.authorTanaka, Aparecida Sadae [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Fed Rio Grande do Sul
dc.date.accessioned2016-01-24T14:38:02Z
dc.date.available2016-01-24T14:38:02Z
dc.date.issued2014-11-01
dc.description.abstractThe Rhipicephalus microplus tick is responsible for losses in the livestock production estimated in 2 billions USD. Despite its economical importance the knowledge in tick's physiology is sparse. in order to contribute to this scenario we describe the characterization of a cysteine proteinase inhibitor named Rmcystatin-3. Purified recombinant Rmcystatin-3 was able to inhibit cathepsin L (Ki = 2.5 nM), BmCl1 (Ki = 1.8 nM) and cathepsin B (Ki = 136 nM). Western blot and quantitative PCR analysis revealed the presence of Rmcystatin-3 in fat body, salivary gland but mainly in hemocytes. the mRNA levels of Rmcystatin-3 during bacterial challenge are drastically down-regulated. in order to define the Rmcystatin-3 possible role in tick immunity, the cystatin gene was knockdown by RNA interference with and without Escherichia coli infection. Our results showed that the Rmcystatin-3 silenced group was more immune competent to control bacterial infection than the group injected with non-related dsRNA. Taking together, our data strongly suggested an important role of Rmcystatin-3 in tick immunity. (C) 2014 Elsevier B.V. and Societe francaise de biochimie et biologie Moleculaire (SFBBM). All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biochem, Escola Paulista Med, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniv Fed Rio Grande do Sul, Ctr Biotecnol Estado Rio Grande do Sul, Porto Alegre, RS, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biochem, Escola Paulista Med, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipINCT-Entomologia Molecular, Brazil
dc.description.sponsorshipIDFAPESP: 05/03514-9
dc.description.sponsorshipIDFAPESP: 09/17589-1
dc.description.sponsorshipIDFAPESP: 12/03657-8
dc.format.extent17-23
dc.identifierhttp://dx.doi.org/10.1016/j.biochi.2014.07.012
dc.identifier.citationBiochimie. Paris: Elsevier France-editions Scientifiques Medicales Elsevier, v. 106C, p. 17-23, 2014.
dc.identifier.doi10.1016/j.biochi.2014.07.012
dc.identifier.fileWOS000344434700003.pdf
dc.identifier.issn0300-9084
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38351
dc.identifier.wosWOS:000344434700003
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBiochimie
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectCystatinen
dc.subjectRNAien
dc.subjectRhipicephalus microplusen
dc.subjectTicksen
dc.subjectInnate immunityen
dc.titleRmcystatin3, a cysteine protease inhibitor from Rhipicephalus microplus hemocytes involved in immune responseen
dc.typeinfo:eu-repo/semantics/article
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