The use of molecular typing to evaluate the dissemination of antimicrobial resistance among gram-negative rods in Brazilian hospitals

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dc.contributor.authorTosin, Iraci [UNIFESP]
dc.contributor.authorSilbert, Suzane [UNIFESP]
dc.contributor.authorSader, Helio Silva [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFederal University of Santa Catarina Centre of Biological Sciences
dc.date.accessioned2015-06-14T13:30:14Z
dc.date.available2015-06-14T13:30:14Z
dc.date.issued2003-12-01
dc.description.abstractAntimicrobial resistance has increased rapidly in Brazil and worldwide during the past few years, giving rise to a growing necessity for antimicrobial resistance surveillance programs. These programs have been instituted in order to monitor bacterial resistance in various regions, and to guide empirical antimicrobial therapy. We evaluated the use of molecular typing in multicenter surveillance programs. We also studied the dissemination modes of selected resistance profiles. Antimicrobial susceptibility to various antimicrobial agents was evaluated by the reference broth microdilution method. Bacterial isolates with selected susceptibility patterns were characterized by pulsed field-gel electrophoresis (PFGE). A total of 119 Gram-negative bacteria were molecularly typed, including 22 imipenem-resistant Pseudomonas aeruginosa, 26 ESBL-producing Escherichia coli, 27 cefoxitin-resistant-ESBL-producing Klebsiella pneumoniae, 33 Enterobacter spp., 8 Citrobacter spp., and 3 S. marcescens isolates resistant to ceftazidime. The isolates were from clinically apparent bacteremia of patients hospitalized in medical centers located in 13 cities of 11 Brazilian states. Our molecular typing results revealed a great genetic diversity among isolates of the same species. However, some major PFGE patterns were found in more than one isolate. All repeated PFGE patterns were detected in only 2 isolates, which were isolated within the same institutions or in different medical centers. We conclude that the ability to characterize organisms phenotypically and genotypically is a powerful epidemiologic tool and it provides unique information that is very important for multicenter surveillance programs.en
dc.description.affiliationFederal University of São Paulo Special Laboratory of Clinical Microbiology
dc.description.affiliationFederal University of Santa Catarina Centre of Biological Sciences
dc.description.affiliationUnifespUNIFESP, Special Laboratory of Clinical Microbiology
dc.description.sourceSciELO
dc.format.extent360-369
dc.identifierhttp://dx.doi.org/10.1590/S1413-86702003000600002
dc.identifier.citationBrazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 7, n. 6, p. 360-369, 2003.
dc.identifier.doi10.1590/S1413-86702003000600002
dc.identifier.fileS1413-86702003000600002.pdf
dc.identifier.issn1413-8670
dc.identifier.scieloS1413-86702003000600002
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/1949
dc.language.isoeng
dc.publisherBrazilian Society of Infectious Diseases
dc.relation.ispartofBrazilian Journal of Infectious Diseases
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMolecular typingen
dc.subjectpulsed-field gel electrophoresisen
dc.subjectantimicrobial resistanceen
dc.subjectcarbapenem-resistant Pseudomonas aeruginosaen
dc.subjectclonal disseminationen
dc.subjectSentinela Programen
dc.titleThe use of molecular typing to evaluate the dissemination of antimicrobial resistance among gram-negative rods in Brazilian hospitalsen
dc.typeinfo:eu-repo/semantics/article
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