Ex vivo assessment of genotoxicity and cytotoxicity in murine fibroblasts exposed to white MTA or white Portland cement with 15% bismuth oxide

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dc.contributor.authorZeferino, Eduardo Gregatto
dc.contributor.authorBueno, Carlos Eduardo da Silveira
dc.contributor.authorOyama, Lila Missae [UNIFESP]
dc.contributor.authorRibeiro, Daniel Araki [UNIFESP]
dc.contributor.institutionSao Leopoldo Mand Univ
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:05:33Z
dc.date.available2016-01-24T14:05:33Z
dc.date.issued2010-10-01
dc.description.abstractP>AimsTo evaluate whether white mineral trioxide aggregate (MTA) or white Portland cement with 15% bismuth oxide were able to induce genetic damage and cellular death ex vivo.MethodologyAliquots of 1 x 104 murine fibroblasts were incubated at 37 degrees C for 3 h with MTA (white) or white Portland cement with 15% bismuth oxide, at final concentrations ranging from 10 to 1000 mu g mL-1 individually. Data of three independent repeats from the comet assay and the trypan blue exclusion test were assessed by the one-way anova followed by Tukey's test.ResultsMineral trioxide aggregate or Portland cement containing bismuth oxide did not produce genotoxic effects with respect to the single-cell gel (comet) assay data for all concentrations evaluated. Furthermore, no cytotoxicity was observed for MTA or Portland cement.ConclusionWhite MTA or white Portland cement containing 15% bismuth oxide were not genotoxic and cytotoxic.en
dc.description.affiliationSao Leopoldo Mand Univ, Dent Res Ctr, Endodont Area, Campinas, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biociencias, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biociencias, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 07/01228-4
dc.description.sponsorshipIDCNPq: 561357/2008-0
dc.format.extent843-848
dc.identifierhttps://dx.doi.org/10.1111/j.1365-2591.2010.01747.x
dc.identifier.citationInternational Endodontic Journal. Malden: Wiley-Blackwell, v. 43, n. 10, p. 843-848, 2010.
dc.identifier.doi10.1111/j.1365-2591.2010.01747.x
dc.identifier.issn0143-2885
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/32972
dc.identifier.wosWOS:000281650700004
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofInternational Endodontic Journal
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectCytotoxicityen
dc.subjectGenotoxicityen
dc.subjectMineral trioxide aggregateen
dc.subjectPortland cementen
dc.titleEx vivo assessment of genotoxicity and cytotoxicity in murine fibroblasts exposed to white MTA or white Portland cement with 15% bismuth oxideen
dc.typeinfo:eu-repo/semantics/article
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