Increased interferon-mediated immunity following in vitro and in vivo Modafinil treatment on peripheral immune cells

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dc.citation.volume81
dc.contributor.authorZager, Adriano
dc.contributor.authorBrandao, Wesley Nogueira
dc.contributor.authorMargatho, Rafael Oliveira
dc.contributor.authorGimenes Cruz, Daniel Sanzio
dc.contributor.authorPeron, Jean Pierre
dc.contributor.authorTufik, Sergio [UNIFESP]
dc.contributor.authorAndersen, Monica Levy [UNIFESP]
dc.contributor.authorMoresco, Monica
dc.contributor.authorPizza, Fabio
dc.contributor.authorPlazzi, Giuseppe
dc.contributor.authorKornum, Birgitte Rahbek
dc.contributor.authorPalermo-Neto, Joao
dc.coverageOxford
dc.date.accessioned2020-07-08T13:09:42Z
dc.date.available2020-07-08T13:09:42Z
dc.date.issued2018
dc.description.abstractThe wake-promoting drug Modafinil has been used for treatment of sleep disorders, such as Narcolepsy, excessive daytime sleepiness and sleep apnea, due to its stimulant action. Despite the known effect of Modafinil on brain neurochemistry, particularly on brain dopamine system, recent evidence support an immunomodulatory role for Modafinil treatment in neuroinflammatory models. Here, we aimed to study the effects of in vitro and in vivo Modafinil treatment on activation, proliferation, cell viability, and cytokine production by immune cells in splenocytes culture from mice. The results show that in vitro treatment with Modafinil increased Interferon (IFN)-gamma, Interleukin (IL)-2 and IL-17 production and CD25 expression by T cells. In turn, in vivo Modafinil treatment enhanced splenocyte production of IFN-gamma, IL-6 and tumor necrosis factor (TNF), and increased the number of IFN-gamma producing cells. Next, we addressed the translational value of the observed effects by testing PBMCs from Narcolepsy type 1 patients that underwent Modafinil treatment. We reported increased number of IFN-gamma producing cells in PBMCs from Narcolepsy type 1 patients following continuous Modafinil treatment, corroborating our animal data. Taken together, our results show, for the first time, a pro-inflammatory action of Modafinil, particularly on IFN-mediated immunity, in mice and in patients with Narcolepsy type 1. The study suggests a novel effect of this drug treatment, which should be taken into consideration when given concomitantly with an ongoing inflammatory or autoimmune process.en
dc.description.affiliationUniv Sao Paulo, Sch Vet Med, Dept Pathol, Neuroimmunomodulat Res Grp, Sao Paulo, Brazil
dc.description.affiliationRigshosp, Mol Sleep Lab, Dept Clin Biochem, Glostrup Res Inst, Glostrup, Denmark
dc.description.affiliationUniv Sao Paulo, Inst Biomed Sci, Dept Immunol, Neuroimmune Interact Lab, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, UNIFESP, EPM, Dept Psychobiol, Sao Paulo, Brazil
dc.description.affiliationUniv Bologna, Dept Biomed & Neuromotor Sci DIBINEM, Bologna, Italy
dc.description.affiliationASL Bologna, IRCCS, Inst Neurol Sci, Bologna, Italy
dc.description.affiliationUnifespUniv Fed Sao Paulo, UNIFESP, EPM, Dept Psychobiol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipSao Paulo State Foundation
dc.description.sponsorshipCNPq
dc.description.sponsorshipAFIP (Associacao Fundo de Incentivo a Pesquisa)
dc.description.sponsorshipIDFAPESP: 2013/18921-5, 2015/18803-8, 2009/51886-3
dc.format.extent297-305
dc.identifierhttp://dx.doi.org/10.1016/j.pnpbp.2017.09.009
dc.identifier.citationProgress In Neuro-Psychopharmacology & Biological Psychiatry. Oxford, v. 81, p. 297-305, 2018.
dc.identifier.doi10.1016/j.pnpbp.2017.09.009
dc.identifier.issn0278-5846
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54146
dc.identifier.wosWOS:000414901100034
dc.language.isoeng
dc.publisherPergamon-Elsevier Science Ltd
dc.relation.ispartofProgress In Neuro-Psychopharmacology & Biological Psychiatry
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectModafinilen
dc.subjectPeripheral immune cellsen
dc.subjectInterferon-gammaen
dc.subjectMiceen
dc.subjectHumanen
dc.subjectNarcolepsy type 1en
dc.titleIncreased interferon-mediated immunity following in vitro and in vivo Modafinil treatment on peripheral immune cellsen
dc.typeinfo:eu-repo/semantics/article
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