Histone Deacetylase 1 Is Essential for Rod Photoreceptor Differentiation by Regulating Acetylation at Histone H3 Lysine 9 and Histone H4 Lysine 12 in the Mouse Retina

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dc.citation.issue6
dc.citation.volume292
dc.contributor.authorFerreira, Renata C. [UNIFESP]
dc.contributor.authorPopova, Evgenya Y.
dc.contributor.authorJames, Jessica
dc.contributor.authorBriones, Marcelo R. S. [UNIFESP]
dc.contributor.authorZhang, Samuel S.
dc.contributor.authorBarnstable, Colin J.
dc.coverageBethesda
dc.date.accessioned2020-07-17T14:02:58Z
dc.date.available2020-07-17T14:02:58Z
dc.date.issued2017
dc.description.abstractHistone acetylation has a regulatory role in gene expression and is necessary for proper tissue development. To investigate the specific roles of histone deacetylases (HDACs) in rod differentiation in neonatal mouse retinas, we used a pharmacological approach that showed that inhibition of class I but not class IIa HDACs caused the same phenotypic changes seen with broad spectrum HDAC inhibitors, most notably a block in the differentiation of rod photoreceptors. Inhibition of HDAC1 resulted in increase of acetylation of lysine 9 of histone 3 (H3K9) and lysine 12 of histone 4 (H4K12) but not lysine 27 of histone 3 (H3K27) and led to maintained expression of progenitor-specific genes such as Vsx2 and Hes1 with concomitant block of expression of rod-specific genes. ChiP experiments confirmed these changes in the promoters of a group of progenitor genes. Based on our results, we suggest that HDAC1-specific inhibition prevents progenitor cells of the retina from exiting the cell cycle and differentiating. HDAC1 may be an essential epigenetic regulator of the transition from progenitor cells to terminally differentiated photoreceptors.en
dc.description.affiliationPenn State Univ, Coll Med, Dept Neural & Behav Sci, 500 Univ Dr, Hershey, PA 17033 USA
dc.description.affiliationPenn State Hershey Eye Ctr, Hershey, PA 17033 USA
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Lab Evolutionary Genom & Biocomplex, BR-04039032 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Lab Evolutionary Genom & Biocomplex, BR-04039032 Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipNational Council for Scientific and Technological Development of Brazil
dc.description.sponsorshipSao Paulo Research Foundation (FAPESP)
dc.description.sponsorshipMacula Vision Research Foundation
dc.description.sponsorshipIDCNPq: 206445/2014-8
dc.description.sponsorshipIDFAPESP: 2013/078380
dc.description.sponsorshipIDFAPESP: 2014/256026
dc.format.extent2422-2440
dc.identifierhttp://dx.doi.org/10.1074/jbc.M116.756643
dc.identifier.citationJournal Of Biological Chemistry. Bethesda, v. 292, n. 6, p. 2422-2440, 2017.
dc.identifier.doi10.1074/jbc.M116.756643
dc.identifier.issn0021-9258
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55106
dc.identifier.wosWOS:000395530300031
dc.language.isoeng
dc.publisherAmer Soc Biochemistry Molecular Biology Inc
dc.relation.ispartofJournal Of Biological Chemistry
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleHistone Deacetylase 1 Is Essential for Rod Photoreceptor Differentiation by Regulating Acetylation at Histone H3 Lysine 9 and Histone H4 Lysine 12 in the Mouse Retinaen
dc.typeinfo:eu-repo/semantics/article
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