Heparin and Hyperbaric Oxygenation in Enteric Autonomic Neuron Preservation for Transplant

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dc.contributor.authorGuimaraes, F. A. G.
dc.contributor.authorTaha, M. O. [UNIFESP]
dc.contributor.authorFagundes, D. J. [UNIFESP]
dc.contributor.authorSimoes, R. S. [UNIFESP]
dc.contributor.authorSimoes, M. J. [UNIFESP]
dc.contributor.authorCaricati-Neto, A. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Santa Cecilia
dc.date.accessioned2016-01-24T13:52:25Z
dc.date.available2016-01-24T13:52:25Z
dc.date.issued2009-04-01
dc.description.abstractBackground. Previous studies have suggested that the addition of heparin to a preservation solution attenuated the autonomic dysfunction observed in rat jejunum and in addition that hypothermic hyperbaric oxygenation may play a role as a preservation technique. However, these studies did not address the lesion indices of the autonomic enteric neurons. We sought to investigate whether the autonomic enteric neurons are injured during cold ischemic preservation and whether administration of heparin or hyperbaric oxygenation prevents this lesion.Methods. Jejunal segments (2 cm; n = 20) of Wistar rats (12-16 weeks old) were maintained in lactated Ringer's solution without or with heparin (H- and H+, respectively) at 4 degrees C under normobaric conditions. Other jejunal segments (n = 10) were maintained at 4 degrees C in H- under hyperbaric oxygenation conditions (HBO). After preservation for 12 hours, H-, H+, and HBO preparations fixed in 10% formaldehyde were stained with hematoxylin and cosin. the lesion indices were expressed as the mean number of affected neurons (karyorhexis, nuclear dislocation, cytoplasmic vacuolisation) per 100 neurons present in intramural ganglia. Statistical analysis was performed using the Mann-Whitney test (P <.05).Results. the histologic studies showed that enteric autonomic neurons were damaged in H- Jejunal segments. the lesion indices observed were: karyorhexis 90/100, nuclear dislocation 85/100, and cytoplasmic vacuolization 82/100. the autonomic neurons in H+ and HBO segments seemed to be normal and significantly well-preserved (P < .001).Conclusion. Hypothermic hyperbaric oxygenation and heparin prevented lesions in cold ischemic preservation of enteric autonomic neurons.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Surg, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Histol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniv Santa Cecilia, Dept Sci, Santos, Brazil
dc.description.affiliationUniv Santa Cecilia, Dept Technol, Santos, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Surg, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Histol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent824-826
dc.identifierhttp://dx.doi.org/10.1016/j.transproceed.2009.02.052
dc.identifier.citationTransplantation Proceedings. New York: Elsevier B.V., v. 41, n. 3, p. 824-826, 2009.
dc.identifier.doi10.1016/j.transproceed.2009.02.052
dc.identifier.issn0041-1345
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31431
dc.identifier.wosWOS:000265504300011
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTransplantation Proceedings
dc.rightsAcesso restrito
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.titleHeparin and Hyperbaric Oxygenation in Enteric Autonomic Neuron Preservation for Transplanten
dc.typeArtigo
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