Testicular morphological changes during the seasonal reproductive cycle in the bullfrog Rana catesbeiana

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dc.contributor.authorSasso-Cerri, E.
dc.contributor.authorDe Faria, F. P.
dc.contributor.authorFreymuller, E.
dc.contributor.authorMiraglia, S. M.
dc.contributor.institutionUniv Nacl Estadual São Paulo
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:34:19Z
dc.date.available2016-01-24T12:34:19Z
dc.date.issued2004-03-01
dc.description.abstractSpermatogenesis and steroidogenesis undergo seasonal variations during the reproductive cycle in amphibians. Testicular morphological and morphometric seasonal variations as well as interstitial lipidic inclusions and intralobular glycoconjugates were evaluated during seasonal cycle of Rana catesbeiana. Testes of frogs collected during the annual seasons were weighed for calculation of GSI (Gonadosomatic index). Seminiferous lobule diameters (DSL) and volume densities of seminiferous lobules (VvSL), excretory ducts (VvED), and interstitial tissue (VvIT) were analyzed. Semithin sections were submitted to Periodic Acid-Schiff (PAS) and Alcian Blue (AB) methods for detection of glycoconjugates, while lipidic inclusions were detected by Sudan Black B. GSI showed no significant variations during the year. Since VvED and VvIT increased significantly during summer and were inversely proportional to VvSL, a compensatory effect between the testicular compartments may be related to the maintenance of GSI. During autumn/winter, larger lobular diameters were observed in comparison to spring/summer when spermiogenesis and spermiation were commonly observed. the increased VvIT and the numerous lipidic inclusions in the interstitial cells during summer suggest a relationship between spermiogenesis and steroidogenesis. Besides the structural stability variations occurring in the IT and SL, a possible paracrine interaction between ED and IT should be also involved in the IT development during summer. the presence of PAS and AB-positive globular structures were observed in the seminiferous lobules and excretory ducts. These structures containing acid glycoconjugates appear to be Sertoli cell apical portions, which are accumulated in the lumen of the seminiferous lobules mainly during spermiation. (C) 2004 Wiley-Liss, Inc.en
dc.description.affiliationUniv Nacl Estadual São Paulo, Dept Morphol, Ctr, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Ctr Electron Microscopy, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Morphol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Ctr Electron Microscopy, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Morphol, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent249-260
dc.identifierhttp://dx.doi.org/10.1002/jez.a.20023
dc.identifier.citationJournal of Experimental Zoology Part A-comparative Experimental Biology. New York: Wiley-liss, v. 301A, n. 3, p. 249-260, 2004.
dc.identifier.doi10.1002/jez.a.20023
dc.identifier.issn0022-104X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27644
dc.identifier.wosWOS:000220055900008
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Experimental Zoology Part A-comparative Experimental Biology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.titleTesticular morphological changes during the seasonal reproductive cycle in the bullfrog Rana catesbeianaen
dc.typeinfo:eu-repo/semantics/article
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