Identification of New Genes Related to Virulence of Xanthomonas axonopodis Pv. Citri during Citrus Host Interactions

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dc.citation.issue1
dc.citation.volume7
dc.contributor.authorFerreira, Cristiano B.
dc.contributor.authorMoreira, Leandro M.
dc.contributor.authorBrigati, Joice B.
dc.contributor.authorLima, Lonjore L.
dc.contributor.authorFerro, Jesus A.
dc.contributor.authorFerro, Maria I. T.
dc.contributor.authorde Oliveira, Julio C. F. [UNIFESP]
dc.coverageIrvine
dc.date.accessioned2020-07-17T14:03:33Z
dc.date.available2020-07-17T14:03:33Z
dc.date.issued2017
dc.description.abstractA mutant library of the bacterium Xanthomonas citri subsp. citri strain 306 pathotype A (Xac), the causative agent of most aggressive Asiatic type A citrus canker, was screened regarding altered canker symptoms after inoculations into Citrus sinensis and Citrus limonia host leaves. Twenty-six mutants have shown phenotypic virulence changes and have respectively knocked out gene identified by sequencing. In vivo growth curves were obtained for nine mutants to quantify how the mutations could affect pathogen's adaptability to growth inside and attack host plant infected tissue. Among identified genes in mutated strains, we could find those that until now had not been reported as being involved in Xac adaptation and/or virulence, such as predicted to encode for xylose repressor-like protein (XAC Delta xylR), Fe-S oxidoredutase (XAC Delta aslB), helicase IV (XAC Delta helD), ubiquinol cytochrome c oxidoreductase iron-sulfur subunit (XAC Delta petA), chromosome partitioning protein (XAC Delta parB) and cell division protein FtsB (XAC Delta ftsB), in addition to genes predicted to encode for hypothetical proteins. The new genes found in this study as being relevant to adaptation and virulence, improve the understanding of Xac fitness during citrus plant attack and canker symptoms development.en
dc.description.affiliationSao Paulo State Univ UNESP, Fac Agr Sci & Vet, Dept Technol, Jaboticabal, SP, Brazil
dc.description.affiliationCitriculture Res Ctr Sylvio Moreira IAC Campinas, Campinas, SP, Brazil
dc.description.affiliationUniv Fed Ouro Preto, Inst Exact & Biol Sci, Dept Biol Sci, Ouro Preto, MG, Brazil
dc.description.affiliationUniv Fed Ouro Preto, Res Ctr Biol Sci NUPEB, Inst Exact & Biol Sci, Dept Biol Sci, Ouro Preto, MG, Brazil
dc.description.affiliationSao Paulo State Univ UNESP, Fac Agr Sci & Vet, Plant Hlth Dept, Jaboticabal, SP, Brazil
dc.description.affiliationFed Univ Sao Paulo UNIFESP, Dept Biol Sci, Diadema, SP, Brazil
dc.description.affiliationUnifespFed Univ Sao Paulo UNIFESP, Dept Biol Sci, Diadema, SP, Brazil
dc.description.provenanceMade available in DSpace on 2020-07-17T14:03:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2017. Added 1 bitstream(s) on 2020-07-17T14:42:50Z : No. of bitstreams: 1 WOS000407171800003.pdf: 6360354 bytes, checksum: a027f46a3d6f8e7c479212477a34168f (MD5)en
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo-FAPESP
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior-CAPES
dc.description.sponsorshipCNPq
dc.description.sponsorshipFundo de Defesa da Citricultura (FUNDECITRUS)
dc.description.sponsorshipCAPES
dc.description.sponsorshipFUNDECITRUS
dc.description.sponsorshipFAPESP
dc.description.sponsorshipIDFAPESP: 04/02006-7
dc.format.extent22-46
dc.identifierhttp://dx.doi.org/10.4236/aim.2017.71003
dc.identifier.citationAdvances In Microbiology. Irvine, v. 7, n. 1, p. 22-46, 2017.
dc.identifier.doi10.4236/aim.2017.71003
dc.identifier.fileWOS000407171800003.pdf
dc.identifier.issn2327-0810
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55488
dc.identifier.wosWOS:000407171800003
dc.language.isoeng
dc.publisherHans Publishers
dc.relation.ispartofAdvances In Microbiology
dc.rightsAcesso aberto
dc.subjectXanthomonas axonopodis Pv. Citrien
dc.subjectMutantsen
dc.subjectImpaired Virulenceen
dc.titleIdentification of New Genes Related to Virulence of Xanthomonas axonopodis Pv. Citri during Citrus Host Interactionsen
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