Tissue-Associated Bacterial Alterations in Rectal Carcinoma Patients Revealed by 16S rRNA Community Profiling

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dc.citation.volume6
dc.contributor.authorThomas, Andrew M.
dc.contributor.authorJesus, Eliane C.
dc.contributor.authorLopes, Ademar
dc.contributor.authorAguiar, Samuel, Jr.
dc.contributor.authorBegnami, Maria D.
dc.contributor.authorRocha, Rafael Malagoli [UNIFESP]
dc.contributor.authorCarpinetti, Paola Avelar
dc.contributor.authorCamargo, Anamaria A.
dc.contributor.authorHoffmann, Christian
dc.contributor.authorFreitas, Helano C.
dc.contributor.authorSilva, Israel T.
dc.contributor.authorNunes, Diana N.
dc.contributor.authorSetubal, Joao C.
dc.contributor.authorDias-Neto, Emmanuel
dc.coverageLausanne
dc.date.accessioned2020-07-31T12:47:04Z
dc.date.available2020-07-31T12:47:04Z
dc.date.issued2016
dc.description.abstractSporadic and inflammatory forms of colorectal cancer (CRC) account for more than 80% of cases. Recent publications have shown mechanistic evidence for the involvement of gut bacteria in the development of both CRC-forms. Whereas, colon and rectal cancer have been routinely studied together as CRC, increasing evidence show these to be distinct diseases. Also, the common use of fecal samples to study microbial communities may reflect disease state but possibly not the tumor microenvironment. We performed this study to evaluate differences in bacterial communities found in tissue samples of 18 rectal-cancer subjects when compared to 18 non-cancer controls. Samples were collected during exploratory colonoscopy (non-cancer group) or during surgery for tumor excision (rectal-cancer group). High throughput 16S rRNA amplicon sequencing of the V4V5 region was conducted on the Ion PGM platform, reads were filtered using Qiime and clustered using UPARSE. We observed significant increases in species richness and diversity in rectal cancer samples, evidenced by the total number of OTUs and the Shannon and Simpson indexes. Enterotyping analysis divided our cohort into two groups, with the majority of rectal cancer samples clustering into one enterotype, characterized by a greater abundance of Bacteroides and Dorea. At the phylum level, rectal-cancer samples had increased abundance of candidate phylum OD1 (also known as Parcubacteria) whilst non-cancer samples had increased abundance of Planctomycetes. At the genera level, rectal-cancer samples had higher abundances of Bacteroides, Phascolarctobacterium, Parabacteroides, Desulfovibrio, and Odoribacter whereas non-cancer samples had higher abundances of Pseudomonas, Escherichia, Acinetobacter, Lactobacillus, and Bacillus. Two Bacteroides fragilis OTUs were more abundant among rectal-cancer patients seen through 16S rRNA amplicon sequencing, whose presence was confirmed by immunohistochemistry and enrichment verified by digital droplet PCR. Our findings point to increased bacterial richness and diversity in rectal cancer, along with several differences in microbial community composition. Our work is the first to present evidence for a possible role of bacteria such as B. fragilis and the phylum Parcubacteria in rectal cancer, emphasizing the need to study tissue-associated bacteria and specific regions of the gastrointestinal tract in order to better understand the possible links between the microbiota and rectal cancer.en
dc.description.affiliationCIPE AC Camargo Canc Ctr, Med Genom Lab, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Cursode Posgrad Bioinformat, Sao Paulo, Brazil
dc.description.affiliationAC Camargo Canc Ctr, Dept Pelv Surg, Sao Paulo, Brazil
dc.description.affiliationAC Camargo Canc Ctr, Dept Pathol, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Coll Med, Dept Gynecol, Lab Mol Gynecol, Sao Paulo, Brazil
dc.description.affiliationHosp Sirio Libane, Ctr Oncol Mol, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Food Res Ctr FoRC, Fac Ciencias Farmaceut, Dept Alimentos & Nutr Expt, Sao Paulo, Brazil
dc.description.affiliationAC Camargo Canc Ctr, Dept Clin Oncol, Sao Paulo, Brazil
dc.description.affiliationAC Camargo Canc Ctr, Lab Computat Biol & Boinformat, Sao Paulo, Brazil
dc.description.affiliationVirginia Tech, Biocomplex Inst, Blacksburg, VA USA
dc.description.affiliationUniv Sao Paulo, Fac Med, Inst Psychiat, Lab Neurosci LIM Alzira Denise Hertzog Silva 27, Sao Paulo, Brazil
dc.description.affiliationUnifespLaboratory of Molecular Gynecology, Department of Gynecology, Medicine College, Universidade Federal de São Paulo (UNIFESP), São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipPrograma Nacional de Apoio à Atenção Oncológica (Pronon)
dc.description.sponsorshipAssociacao Beneficiente Alzira Denise Hertzog Silva (ABADHS)
dc.description.sponsorshipIDFAPESP: 2015/01507-7
dc.description.sponsorshipIDFAPESP: 2013/07914-8
dc.description.sponsorshipIDCAPES: 88887.062078/2014-00
dc.description.sponsorshipIDCAPES: 3385/2013
dc.description.sponsorshipIDPRONON: 25000.055.167/2015-23
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.3389/fcimb.2016.00179
dc.identifier.citationFrontiers In Cellular And Infection Microbiology. Lausanne, v. 6, p. -, 2016.
dc.identifier.doi10.3389/fcimb.2016.00179
dc.identifier.fileWOS000389614300001.pdf
dc.identifier.issn2235-2988
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56556
dc.identifier.wosWOS:000389614300001
dc.language.isoeng
dc.publisherFrontiers Media Sa
dc.relation.ispartofFrontiers In Cellular And Infection Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectmucosa-associated microbiotaen
dc.subjectrectal canceren
dc.subject16S rRNA gene sequencingen
dc.subjectBacteroides fragilisen
dc.subjectBacterial diversity and community compositionen
dc.titleTissue-Associated Bacterial Alterations in Rectal Carcinoma Patients Revealed by 16S rRNA Community Profilingen
dc.typeinfo:eu-repo/semantics/article
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