Ultrastructural characterization of CD133(+) stem cells bound to superparamagnetic nanoparticles: possible biotechnological applications

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dc.contributor.authorPavon, L. F.
dc.contributor.authorGamarra, L. F.
dc.contributor.authorMarti, L. C.
dc.contributor.authorAmaro Junior, E.
dc.contributor.authorMoreira-Filho, C. A.
dc.contributor.authorCamargo-Mathias, M. I.
dc.contributor.authorOkamoto, Oswaldo Keith [UNIFESP]
dc.contributor.institutionAlbert Einstein Res & Educ Inst IEPAE
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUNESP
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:51:38Z
dc.date.available2016-01-24T13:51:38Z
dc.date.issued2008-09-01
dc.description.abstractCD133 antigen is an integral membrane glycoprotein that can bind with different cells. Originally, however. this cellular surface antigen was expressed in human stem cells and in various cellular progenitors of the haematopoietic system. Human cord blood has been described as an excellent source of CD133(+) haematopoietic progenitor cells with a large application potential. One of the main objectives of the present study is to describe for the first time the ultrastructural characteristics of CD133(+) stem cells using transmission electronic microscopy. Another objective of the manuscript is to demonstrate through transmission electronic microscopy the molecular image of magnetic nanoparticles connected to the stein cells of great biotechnological importance, as well as demonstrating the value of this finding for electronic paramagnetic resonance and its related nanobioscientific value. Ultrastructural results showed the monoclonal antibody anti-CD133 bound to the superparamagnetic nanoparticles by the presence of electrondense granules in cell membrane, as well as in the cytoplasm, revealing the ultrastructural characteristics of CD133(+) cells, exhibiting a round morphology with discrete cytoplasmic projections, having an active nucleus that follows this morphology. the cellular cytoplasm was filled up with mitochondrias, as well as microtubules and vesicles pinocitic. characterizing the process as being related to internalization of the magnetic nanoparticles that were endocyted by the cells in question. Electronic paramagnetic resonance analysis of the CD133(+) stem cells detected that the small (spectrum) generated by the labelled cells comes from the superparamagnetic nanoparticles that are bound to them. These results strongly suggest that these CD133(+) cells can be used in nanobiotechnology applications, with benefits in different biomedical areas.en
dc.description.affiliationAlbert Einstein Res & Educ Inst IEPAE, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Biotechnol Program, IPT, Butanan Inst, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Radiol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Immunol, Inst Biomed Sci, São Paulo, Brazil
dc.description.affiliationUNESP, Biosci Inst, Dept Biol, Rio Claro, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Neurol & Neurosurg, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Neurol & Neurosurg, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipSociedade Beneficente Israelita Brasileira Hospital Albert Einstein SBIBHAE
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDSociedade Beneficente Israelita Brasileira Hospital Albert Einstein SBIBHAE: (IIEP-AE 278-07
dc.description.sponsorshipIDFAPESP: 02/01826-5
dc.format.extent374-383
dc.identifierhttp://dx.doi.org/10.1111/j.1365-2818.2008.02049.x
dc.identifier.citationJournal of Microscopy-oxford. Oxford: Blackwell Publishing, v. 231, n. 3, p. 374-383, 2008.
dc.identifier.doi10.1111/j.1365-2818.2008.02049.x
dc.identifier.issn0022-2720
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/30864
dc.identifier.wosWOS:000259611000003
dc.language.isoeng
dc.publisherBlackwell Publishing
dc.relation.ispartofJournal of Microscopy-oxford
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectmagnetic nanoparticlesen
dc.subjectnanobiotechnologyen
dc.subjectstem cellsen
dc.subjectultrastructureen
dc.titleUltrastructural characterization of CD133(+) stem cells bound to superparamagnetic nanoparticles: possible biotechnological applicationsen
dc.typeinfo:eu-repo/semantics/article
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