Mastoparan induces apoptosis in B1 6F10-Nex2 melanoma cells via the intrinsic mitochondrial pathway and displays antitumor activity in vivo

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dc.contributor.authorAzevedo, Ricardo A. de
dc.contributor.authorFigueiredo, Carlos R. [UNIFESP]
dc.contributor.authorFerreira, Adilson K.
dc.contributor.authorMatsuo, Alisson L. [UNIFESP]
dc.contributor.authorMassaoka, Mariana H. [UNIFESP]
dc.contributor.authorGirola, Natalia [UNIFESP]
dc.contributor.authorAuada, Aline V. V.
dc.contributor.authorFarias, Camyla F. [UNIFESP]
dc.contributor.authorPasqualoto, Kerly F. M.
dc.contributor.authorRodrigues, Cecilia P.
dc.contributor.authorBarbuto, Jose A.
dc.contributor.authorLevy, Debora
dc.contributor.authorBydlowski, Sergio P.
dc.contributor.authorSa-Junior, Paulo L. de
dc.contributor.authorTravassos, Luiz R. [UNIFESP]
dc.contributor.authorLebrun, Ivo
dc.contributor.institutionButantan Inst
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:40:33Z
dc.date.available2016-01-24T14:40:33Z
dc.date.issued2015-06-01
dc.description.abstractMastoparan is an a-helical and amphipathic tetradecapeptide obtained from the venom of the wasp Vespula lewisii. This peptide exhibits a wide variety of biological effects, including antimicrobial activity, increased histamine release from mast cells, induction of a potent mitochondrial permeability transition and tumor cell cytotoxicity. Here, the effects of mastoparan in malignant melanoma were studied using the murine model of B16F10-Nex2 cells. in vitro, mastoparan caused melanoma cell death by the mitochondrial apoptosis pathway, as evidenced by the Annexin V-FITC/PI assay, loss of mitochondrial membrane potential (Delta psi(m)), generation of reactive oxygen species, DNA degradation and cell death signaling. Most importantly, mastoparan reduced the growth of subcutaneous melanoma in syngeneic mice and increased their survival. the present results show that mastoparan induced caspase-dependent apoptosis in melanoma cells through the intrinsic mitochondrial pathway protecting the mice against tumor development. (C) 2014 Elsevier Inc. All rights reserved.en
dc.description.affiliationButantan Inst, Biochem & Biophys Lab, São Paulo, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Expt Oncol Unit UNONEX, São Paulo, SP, Brazil
dc.description.affiliationUniv São Paulo, Inst Biomed Sci, Dept Immunol, BR-05508 São Paulo, SP, Brazil
dc.description.affiliationUniv São Paulo, Cell & Mol Therapy Ctr NUCEL NETCEM, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Med, Lab Genet & Mol Hematol LIM31, BR-05508 São Paulo, Brazil
dc.description.affiliationButantan Inst, Lab Genet, São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Expt Oncol Unit UNONEX, São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipINCT Tox
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 2010/51077-5
dc.format.extent113-119
dc.identifierhttp://dx.doi.org/10.1016/j.peptides.2014.09.024
dc.identifier.citationPeptides. New York: Elsevier B.V., v. 68, p. 113-119, 2015.
dc.identifier.doi10.1016/j.peptides.2014.09.024
dc.identifier.issn0196-9781
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/39130
dc.identifier.wosWOS:000354572200016
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofPeptides
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectMastoparanen
dc.subjectApoptosisen
dc.subjectIntrinsic pathwayen
dc.subjectMelanomaen
dc.subjectAntitumor effects in vivoen
dc.titleMastoparan induces apoptosis in B1 6F10-Nex2 melanoma cells via the intrinsic mitochondrial pathway and displays antitumor activity in vivoen
dc.typeinfo:eu-repo/semantics/article
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