Lateral hypothalamic serotenergic responsiveness to food intake in rat obesity as measured by microdialysis

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dc.contributor.authorMori, Rosana Cristina Tieko [UNIFESP]
dc.contributor.authorGuimaraes, Regina Barros [UNIFESP]
dc.contributor.authorNascimento, Claudia Maria da Penha Oller do [UNIFESP]
dc.contributor.authorRibeiro, Eliane Beraldi [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:30:47Z
dc.date.available2016-01-24T12:30:47Z
dc.date.issued1999-04-01
dc.description.abstractThe hypothalamic serotonergic system is involved in the regulation of food ingestion and energy metabolism. Since disturbances of both energy intake and expenditure can contribute to obesity, the objective of the present study was to evaluate the serotonergic response stimulated by food ingestion in two different models of obesity: the hyperphagic Zucker and the hypophagic and hypometabolic, monosodium glutamate (MSG) obese Wistar rat. for this we used microdialysis to examine the release of 5-hydroxytryptamine (serotonin, 5HT) and 5-hydroxyindoleacetic acid (5HIAA) in the lateral hypothalamus. Daily intake of MSG-obese rats was 40% lower while that of Zucker obese rats was 60% higher than that of the respective lean controls. in overnight-fasted animals, 20-min microdialysate samples were collected before (basal release) and during a 2-h period of access to a balanced palatable food mash. the animals began to eat during the first 20 min of food access, and food consumption was similar among the four groups in all six individual 20-min periods recorded. Ingestion of food increased 5HT release in all groups. in MSG-obese and lean Wistar rats, 5HT levels were similarly elevated during the whole experimental period. in the Zucker strain, 5HT increments of basal release tended to be higher in obese than in lean rats at 20 and 40 min, and a significantly higher increment was observed at 60 min after food access (40 and 135% for lean and obese, respectively). the area under the curve relating serotonin levels to the 120 min of food availability was significantly higher in Zucker obese (246.7 +/- 23.3) than MSG-obese (152.7 +/- 13.4), lean Wistar (151.9 +/- 11.1), and lean Zucker (173.5 +/- 24.0) rats. the present observation, of a food-induced serotonin release in the lateral hypothalamus of lean Wistar and Zucker rats, evidences that 5HT in the lateral hypothalamus is important in the normal response to feeding. in obese animals, the serotonin response was similar to (in the hypophagic-hypometabolic MSG model) or even higher than (in the hyperphagic Zucker model) that seen in the respective lean controls. This result indicates that the energy homeostasis disturbances of both these obesity models may not be ascribed to an impairment of the acute lateral hypothalamic serotonin response to a dietary stimulus.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Physiol, Div Neurophysiol & Endocrin Physiol, BR-04023060 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Physiol, Div Neurophysiol & Endocrin Physiol, BR-04023060 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent286-292
dc.identifierhttps://dx.doi.org/10.1139/cjpp-77-4-286
dc.identifier.citationCanadian Journal of Physiology and Pharmacology. Ottawa: Natl Research Council Canada, v. 77, n. 4, p. 286-292, 1999.
dc.identifier.doi10.1139/cjpp-77-4-286
dc.identifier.issn0008-4212
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/26050
dc.identifier.wosWOS:000081297400007
dc.language.isoeng
dc.publisherNatl Research Council Canada
dc.relation.ispartofCanadian Journal of Physiology and Pharmacology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSerotoninen
dc.subjectFood intakeen
dc.subjectBrain microdialysisen
dc.subjectLateral hypothalamusen
dc.subjectMonosodium glutamate obesityen
dc.subjectZucker obesityen
dc.titleLateral hypothalamic serotenergic responsiveness to food intake in rat obesity as measured by microdialysisen
dc.typeinfo:eu-repo/semantics/article
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