Sentinel lymph node biopsy in rats. Comparison between paraffin and frozen section analysis

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dc.contributor.authorSantos Filho, Ivan Dunshee de Abranches Oliveira [UNIFESP]
dc.contributor.authorEnokihara, Mílvia Maria Simões e Silva [UNIFESP]
dc.contributor.authorShie, Janaina Namba [UNIFESP]
dc.contributor.authorOliveira, Andrea Fernandes de [UNIFESP]
dc.contributor.authorSantos, Ivan Dunshee de Abranches Oliveira [UNIFESP]
dc.contributor.authorFerreira, Lydia Masako [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:46:53Z
dc.date.available2015-06-14T13:46:53Z
dc.date.issued2014-01-01
dc.description.abstractPURPOSE:To simulate a lymph node metastasis in an animal model using activated carbon, assess their identification in frozen section analysis and compare with histopathological examination in paraffin.METHODS:Thirty two adult female rats were used. They received the carbon injection on its hind legs. Half of the rats was sacrificed on day one, and the other half after 21 days. Thus, 64 lymph nodes were dissected and split longitudinally. One half of the lymph node was sent immediately to frozen section analysis. The other half was fixed in 10% formaldehyde to be cut in paraffin. Slides were divided into quadrants and classified by the presence of carbon in these four quadrants_ They were also classified by the carbon staining intensity.RESULTS:Comparing the slides obtained in the first day and 21 days, there was a tendency of carbon to spread over time, but without statistical significance. The intensity did not alter over time.CONCLUSION:There was no concordance between the two methods of pathological analysis, however the actived carbon was seen in all lymph nodes.en
dc.description.affiliationUNIFESP
dc.description.affiliationUNIFESP Pathology Department
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Department of Surgery
dc.description.affiliationUnifespUNIFESP, Pathology Department
dc.description.affiliationUnifespUNIFESP, Department of Surgery
dc.description.sourceSciELO
dc.format.extent57-61
dc.identifierhttp://dx.doi.org/10.1590/S0102-86502014001300011
dc.identifier.citationActa Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 57-61, 2014.
dc.identifier.doi10.1590/S0102-86502014001300011
dc.identifier.fileS0102-86502014001300012.pdf
dc.identifier.issn0102-8650
dc.identifier.scieloS0102-86502014001300012
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/8197
dc.identifier.wosWOS:000342065000012
dc.language.isoeng
dc.publisherSociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartofActa Cirurgica Brasileira
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectLymph node biopsyen
dc.subjectFrozen sectionsen
dc.subjectParaffin embeddingen
dc.subjectCharcoalen
dc.subjectRatsen
dc.titleSentinel lymph node biopsy in rats. Comparison between paraffin and frozen section analysisen
dc.typeinfo:eu-repo/semantics/article
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