Differential expression of Ro/SSA 60 kDa and La/SSB, but not Ro/SSA 52 kDa, mRNA and protein in minor salivary glands from patients with primary Sjogren's syndrome

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dc.contributor.authorBarcellos, Karin S. A. [UNIFESP]
dc.contributor.authorNonogaki, Suely [UNIFESP]
dc.contributor.authorEnokihara, Milvia M. S. S. [UNIFESP]
dc.contributor.authorTeixeira, Miriam S. [UNIFESP]
dc.contributor.authorAndrade, Luis E. C. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionAdolpho Lutz Inst
dc.date.accessioned2018-06-18T10:46:12Z
dc.date.available2018-06-18T10:46:12Z
dc.date.issued2007-06-01
dc.description.abstractObjective. To analyze the protein and messenger RNA (mRNA) expression of La/SSB, Ro/SSA 60, and Ro/SSA 52 antigens in minor salivary glands (MSG) from patients with primary Sjogren's syndrome (pSS).Methods. La/SSB, Ro/SSA 60, and Ro/SSA 52 protein expression was studied by immunohistochemistry in MSG from 26 patients with pSS and 16 controls. mRNA expression was determined by real-time polymerase chain reaction in MSG of 10 patients with pSS and 7 controls.Results. La/SSB and Ro/SSA 60, but not Ro/SSA 52, mRNA expression was higher in samples from patients with pSS compared to controls (p < 0.05). La/SSB protein had higher expression in the cytoplasm of ductal cells than in the cytoplasm of mucous acinar cells in patients with pSS (p = 0.013) but not in controls. Ro/SSA 60 had higher expression in the cytoplasm of ductal cells than in the cytoplasm of serous acinar cells in patients with pSS (p = 0.006) but not in controls. The Ro/SSA 52 expression pattern was similar in patients and controls. There was no association between circulating autoantibodies to Ro/SSA or La/SSB and the aberrant expression of the cognate autoantigens.Conclusion. The increased Ro/SSA 60 and La/SSB mRNA expression in MSG of patients with pSS as well as the differential Ro/SSA 60 and La/SSB protein expression in ductal cells of MSG in patients with pSS suggest that these these 2 autoantigens, but not Ro/SSA 52, are probably involved in triggering and maintaining the tissue-specific autoimmune response in pSS MSG and may contribute to the antigen-driven immune response and local autoantibody productionen
dc.description.affiliationUniv Fed Sao Paulo, Div Rheumatol, BR-04023062 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Div Pathol, BR-04023062 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Stomatol Div, BR-04023062 Sao Paulo, Brazil
dc.description.affiliationAdolpho Lutz Inst, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Div Rheumatol, BR-04023062 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Div Pathol, BR-04023062 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Stomatol Div, BR-04023062 Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent1283-1292
dc.identifierhttp://www.jrheum.org/content/34/6/1283
dc.identifier.citationJournal Of Rheumatology. Toronto: J Rheumatol Publ Co, v. 34, n. 6, p. 1283-1292, 2007.
dc.identifier.issn0315-162X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/44682
dc.identifier.wosWOS:000247116600014
dc.language.isoeng
dc.publisherJ Rheumatol Publ Co
dc.relation.ispartofJournal Of Rheumatology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectSjogren's syndromeen
dc.subjectmRNA expressionen
dc.subjectautoantibodiesen
dc.subjectminor salivary glandsen
dc.titleDifferential expression of Ro/SSA 60 kDa and La/SSB, but not Ro/SSA 52 kDa, mRNA and protein in minor salivary glands from patients with primary Sjogren's syndromeen
dc.typeinfo:eu-repo/semantics/article
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