Effect of antibodies to intercellular adhesion molecule type 1 on the protection of distant organs during reperfusion syndrome in rats

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dc.contributor.authorSouza-Moraes, M.r. [UNIFESP]
dc.contributor.authorDavid-Filho, R. [UNIFESP]
dc.contributor.authorBaptista-Silva, José Carlos Costa [UNIFESP]
dc.contributor.authorUllian, M. [UNIFESP]
dc.contributor.authorFranco, Marcello Fabiano de [UNIFESP]
dc.contributor.authorGabriel Jr., A. [UNIFESP]
dc.contributor.authorSmith, B. [UNIFESP]
dc.contributor.authorBurihan, E. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:30:00Z
dc.date.available2015-06-14T13:30:00Z
dc.date.issued2003-05-01
dc.description.abstractWe investigated kidney and lung alterations caused by intercellular adhesion molecule type 1 (ICAM-1) blockade after ischemia and reperfusion of hind limb skeletal muscles. Rats were submitted to ligature of the infrarenal aorta for 6 h. The animals were randomized into three groups of 6 rats each: group I, sacrificed after ischemia; group II, reperfusion for 24 h, and group III, reperfusion for 24 h after receiving monoclonal anti-ICAM-1 antibodies. At the end of the experiment, blood samples were collected for creatinine, lactate dehydrogenase, creatine phosphokinase, potassium, pH and leukocyte counts. Samples were taken from the muscles of the hind limbs and from the kidneys and lungs for histological analysis and measurement of the neutrophil infiltrate by myeloperoxidase staining. The groups did not differ significantly with regard to the laboratory tests. There were no major histological alterations in the kidneys. An intense neutrophil infiltrate in the lungs, similar in all groups, was detected. Myeloperoxidase determination showed that after reperfusion there was significantly less retention of polymorphonuclear neutrophils in the muscles (352 ± 70 vs 1451 ± 235 × 10² neutrophils/mg; P<0.01) and in the kidneys (526 ± 89 vs 852 ± 73 × 10² neutrophils/mg; P<0.01) of the animals that received anti-ICAM-1 before perfusion compared to the group that did not. The use of anti-ICAM-1 antibodies in this experimental model minimized neutrophil influx, thus reducing the inflammatory process, in the muscles and kidneys after ischemia and reperfusion of the hind limbs.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Cirurgia
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Clínica
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Patologia
dc.description.affiliationUnifespUNIFESP, EPM, Depto. de Cirurgia
dc.description.affiliationUnifespUNIFESP, EPM, Depto. de Clínica
dc.description.affiliationUnifespUNIFESP, EPM, Depto. de Patologia
dc.description.sourceSciELO
dc.format.extent605-612
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2003000500007
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 5, p. 605-612, 2003.
dc.identifier.doi10.1590/S0100-879X2003000500007
dc.identifier.fileS0100-879X2003000500007.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2003000500007
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/1711
dc.identifier.wosWOS:000183100000007
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectICAM-1en
dc.subjectNeutrophilen
dc.subjectMyeloperoxidaseen
dc.subjectKidneyen
dc.subjectLungen
dc.subjectReperfusionen
dc.titleEffect of antibodies to intercellular adhesion molecule type 1 on the protection of distant organs during reperfusion syndrome in ratsen
dc.typeinfo:eu-repo/semantics/article
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