Differential role of the estrogen receptors ESR1 and ESR2 on the regulation of proteins involved with proliferation and differentiation of Sertoli cells from 15-day-old rats

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dc.contributor.authorLucas, Thais Fabiana Gameiro [UNIFESP]
dc.contributor.authorLazari, Maria de Fatima Magalhaes [UNIFESP]
dc.contributor.authorPorto, Catarina Segreti [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:35:10Z
dc.date.available2016-01-24T14:35:10Z
dc.date.issued2014-01-25
dc.description.abstractThe aim of the present study was to investigate the role of each estrogen receptors on the regulation of proteins involved with proliferation and differentiation of Sertoli cells from 15-day-old rats. Activation of ESR1 by 17 beta-estradiol (E2) and ESR1-selective agonist PPT increased CCND1 expression, and this effect was dependent on NF-kB activation. E2 and the ESR2-selective agonist DPN, but not PPT, increased, in a PI3K and CREB-dependent manner, the expression of CDKN1B and the transcription factors GATA-1 and DMRT1. Analyzing the expression of ESR1 and ESR2 in different stages of development of Sefton cells, we observed that the ESR1/ESR2 ratio decreased with age, and this ratio seems to be important to determine the end of cell proliferation and the start of cell differentiation. in Sertoli cells from 15-day-old rats, the ESR1/ESR2 ratio favors the effect of ESR1 and the activation of this receptor increased [Methyl-31-I]thymidine incorporation. We propose that in Sertoli cells from 15-day-old rats E2 modulates Sertoli cell proliferation through ESR1/NF-kappa B-mediated increase of CCND1, and cell cycle exit and differentiation through ESR2/CREB-mediated increase of CDKN1B, GATA-1 and DMRT1. the present study reinforces the important role of estrogen for normal testis development. (C) 2013 Elsevier Ireland Ltd. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol,INFAR, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol,INFAR, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipIDFAPESP: 2010/52306-8pt
dc.format.extent84-96
dc.identifierhttps://dx.doi.org/10.1016/j.mce.2013.09.015
dc.identifier.citationMolecular and Cellular Endocrinology. Clare: Elsevier B.V., v. 382, n. 1, p. 84-96, 2014.
dc.identifier.doi10.1016/j.mce.2013.09.015
dc.identifier.issn0303-7207
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/37328
dc.identifier.wosWOS:000330421600010
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMolecular and Cellular Endocrinology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectEstrogen receptorsen
dc.subjectSertoli cellsen
dc.subjectCCND1en
dc.subjectCDKN1Ben
dc.subjectGATA-1en
dc.subjectDMRT1en
dc.titleDifferential role of the estrogen receptors ESR1 and ESR2 on the regulation of proteins involved with proliferation and differentiation of Sertoli cells from 15-day-old ratsen
dc.typeinfo:eu-repo/semantics/article
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