Chitosan nanoparticles for nitric oxide delivery in human skin

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dc.citation.issue4
dc.citation.volume8
dc.contributor.authorPelegrino, M. T. [UNIFESP]
dc.contributor.authorWeller, R. B.
dc.contributor.authorChen, X.
dc.contributor.authorBernardes, J. S.
dc.contributor.authorSeabra, A. B.
dc.coverageCambridge
dc.date.accessioned2020-07-17T14:02:27Z
dc.date.available2020-07-17T14:02:27Z
dc.date.issued2017
dc.description.abstractThe use of nanoparticle-based transdermal delivery systems is a promising approach to efficiently carry and deliver therapeutic agents for dermal and systemic administration. Nitric oxide (NO) is a key molecule that plays important roles in human skin such as the control of skin homeostasis, skin defense, control of dermal blood flow, and wound healing. In addition, human skin contains stores of NO derivatives that can be mobilized and release free NO upon UV irradiation with beneficial cardiovascular effects, for instance the control of blood pressure. In this work, the NO donor precursor glutathione (GSH) was encapsulated (encapsulation efficiency of 99.60%) into ultra-small chitosan nanoparticles (CS NPs) (hydrodynamic size of 30.65 +/- 11.90 nm). GSH-CS NPs have a core-shell structure, as revealed by atomic force microscopy and X-ray photoelectron spectroscopy, in which GSH is protected in the nanoparticle core. Nitrosation of GSH by nitrous acid led to the formation of the NO donor S-nitrosogluthathione (GSNO) into CS NPs. The GSNO release from the CS NPs followed a Fickian diffusion described by the Higuchi mathematical model. Topical application of GSNO-CS NPs in intact human skin significantly increased the levels of NO and its derivatives in the epidermis, as assayed by confocal microscopy, and this effect was further enhanced by skin irradiation with UV light. Therefore, NO-releasing CS NPs are suitable materials for transdermal NO delivery to local and/or systemic therapies.en
dc.description.affiliationUniv Fed Sao Paulo, Exact & Earth Sci Dept, Rua Sao Nicolau 210, BR-09913030 Diadema, SP, Brazil
dc.description.affiliationUniv Fed ABC, Ctr Nat & Human Sci, Av Estados 5001, BR-09210580 Santo Andre, SP, Brazil
dc.description.affiliationUniv Edinburgh, Queens Med Res Inst, MRC, Ctr Inflammat Res, 47 Little France Crescent, Edinburgh EH16 4TJ, Midlothian, Scotland
dc.description.affiliationNatl Ctr Energy & Mat CNPEM, Natl Nanotechnol Lab LNNano, Rua Giuseppe Maximo Scolfaro 10-000, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Exact & Earth Sci Dept, Rua Sao Nicolau 210, BR-09913030 Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPESP [2015/00393-8, 2016/10347-6]
dc.description.sponsorshipBrazilian Network on Nanotoxicology (MCTI/CNPq) [552120/2011-1]
dc.description.sponsorshipLaboratory of Nanostructure Synthesis and Biosystem InteractionsNANOBIOSS (MCTI) [402280-2013]
dc.description.sponsorshipNewton Advanced Fellowship (The Royal Society) [NA140046]
dc.format.extent713-719
dc.identifierhttp://dx.doi.org/10.1039/c6md00502k
dc.identifier.citationMedchemcomm. Cambridge, v. 8, n. 4, p. 713-719, 2017.
dc.identifier.doi10.1039/c6md00502k
dc.identifier.issn2040-2503
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54816
dc.identifier.wosWOS:000399918800002
dc.language.isoeng
dc.publisherRoyal Soc Chemistry
dc.relation.ispartofMedchemcomm
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.titleChitosan nanoparticles for nitric oxide delivery in human skinen
dc.typeinfo:eu-repo/semantics/article
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