Escherichia albertii, a novel human enteropathogen, colonizes rat enterocytes and translocates to extra-intestinal sites

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dc.citation.issue2
dc.citation.volume12
dc.contributor.authorYamamoto, Denise [UNIFESP]
dc.contributor.authorHernandes, Rodrigo T. [UNIFESP]
dc.contributor.authorLiberatore, Ana Maria A. [UNIFESP]
dc.contributor.authorAbe, Cecilia M.
dc.contributor.authorde Souza, Rodrigo B. [UNIFESP]
dc.contributor.authorRomao, Fabiano T. [UNIFESP]
dc.contributor.authorSperandio, Vanessa
dc.contributor.authorKoh, Ivan H. [UNIFESP]
dc.contributor.authorGomes, Tania A. T. [UNIFESP]
dc.coverageSan Francisco
dc.date.accessioned2020-07-17T14:02:58Z
dc.date.available2020-07-17T14:02:58Z
dc.date.issued2017
dc.description.abstractDiarrhea is the second leading cause of death of children up to five years old in the developing countries. Among the etiological diarrheal agents are atypical enteropathogenic Escherichia coli (aEPEC), one of the diarrheagenic E. coli pathotypes that affects children and adults, even in developed countries. Currently, genotypic and biochemical approaches have helped to demonstrate that some strains classified as aEPEC are actually E. albertii, a recently recognized human enteropathogen. Studies on particular strains are necessary to explore their virulence potential in order to further understand the underlying mechanisms of E. albertii infections. Here we demonstrated for the first time that infection of fragments of rat intestinal mucosa is a useful tool to study the initial steps of E. albertii colonization. We also observed that an E. albertii strain can translocate from the intestinal lumen to Mesenteric Lymph Nodes and liver in a rat model. Based on our finding of bacterial translocation, we investigated how E. albertii might cross the intestinal epithelium by performing infections of M-like cells in vitro to identify the potential in vivo translocation route. Altogether, our approaches allowed us to draft a general E. albertii infection route from the colonization till the bacterial spreading in vivo.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med UNIFESP EPM, Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Dept Microbiol & Imunol, Inst Biociencia, Botucatu, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Cirurgia, Escola Paulista Med UNIFESP EPM, Sao Paulo, Brazil
dc.description.affiliationInst Butantan, Lab Biol Celular, Sao Paulo, Brazil
dc.description.affiliationUniv Texas Southwestern Med Ctr Dallas, Dept Microbiol & Biochem, Dallas, TX 75390 USA
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med UNIFESP EPM, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Cirurgia, Escola Paulista Med UNIFESP EPM, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFUNDACAO DE AM PARO A PESQUISA DO ESTADO DE SAO PAULO (FAPESP)
dc.description.sponsorshipCOORDENACAO DE APERFEICOAMENTO DE PESSOAL DE NIVEL SUPERIOR (CAPES)
dc.description.sponsorshipIDFAPESP: 2011/12664-5
dc.description.sponsorshipIDCAPES: AUX-PE-PNPD 2350/2011
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0171385
dc.identifier.citationPlos One. San Francisco, v. 12, n. 2, p. -, 2017.
dc.identifier.doi10.1371/journal.pone.0171385
dc.identifier.fileWOS000393712500039.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55113
dc.identifier.wosWOS:000393712500039
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleEscherichia albertii, a novel human enteropathogen, colonizes rat enterocytes and translocates to extra-intestinal sitesen
dc.typeinfo:eu-repo/semantics/article
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