Evaluating and Improving Vitek MS for Identification of Clinically Relevant Species of Trichosporon and the Closely Related Genera Cutaneotrichosporon and Apiotrichum

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dc.contributor.authorde Almeida, Joao N., Jr.
dc.contributor.authorFavero Gimenes, Viviane M.
dc.contributor.authorFrancisco, Elaine C. [UNIFESP]
dc.contributor.authorMachado Siqueira, Lumena P.
dc.contributor.authorGoncalves de Almeida, Renato K.
dc.contributor.authorGuitard, Juliette
dc.contributor.authorHennequin, Christophe
dc.contributor.authorColombo, Arnaldo L. [UNIFESP]
dc.contributor.authorBenard, Gil
dc.contributor.authorRossi, Flavia
dc.date.accessioned2019-08-19T11:49:56Z
dc.date.available2019-08-19T11:49:56Z
dc.date.issued2017
dc.description.abstractTrichosporon species are relevant etiologic agents of hospital-acquired infections. High mortality rates are attributed to Trichosporon deep-seated infections in immuno-compromised individuals, making fast and accurate species identification relevant for hastening the discovery of best-targeted therapy. Recently, Trichosporon taxonomy has been reassessed, and three genera have been proposed for the pathogenic species: Trichosporon, Cutaneotrichosporon, and Apiotrichum. Matrix-assisted laser desorption ionization- time of flight mass spectrometry (MALDI-TOF MS) has replaced old phenotypic methods for microorganism identification in clinical laboratories, but spectral profile databases have to be evaluated and improved for optimal species identification performance. Vitek MS (bioMerieux) is one of the commercially available MALDI-TOF MS platforms for pathogen identification, and its spectral profile databases remain poorly evaluated for Trichosporon, Cutaneotrichosporon, and Apiotrichum species identification. We herein evaluated and improved Vitek MS for the identification of the main clinical relevant species of Trichosporon, Cutaneotrichosporon, and Apiotrichum using a large set of strains and isolates belonging to different yeast collections in Brazil and France.en
dc.description.affiliationUniv São Paulo, Fac Med, Hosp Clin, Cent Lab Div LIM03, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Med Trop São Paulo, Lab Mycol LIM 53, São Paulo, Brazil
dc.description.affiliationUniv Fed São Paulo, Escola Paulista Med, Lab Especial Micol, São Paulo, Brazil
dc.description.affiliationHop St Antoine, AP HP, Serv Parasitol Mycol, Paris, France
dc.description.affiliationUPMC Univ Paris 06, INSERM, CNRS, UMR 1135,Sorbonne Univ,ERL 8255, Paris, France
dc.description.affiliationCtr Immunol & Malad Infect CIMI Paris, Paris, France
dc.description.affiliationUnifespUniv Fed São Paulo, Escola Paulista Med, Lab Especial Micol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipNational Council of Technological and Scientific Development (CNPq)
dc.description.sponsorshipIDCNPq: 307510/2015-8
dc.format.extent2439-2444
dc.identifierhttp://dx.doi.org/10.1128/JCM.00461-17
dc.identifier.citationJournal Of Clinical Microbiology. Washington, v. 55, n. 8, p. 2439-2444, 2017.
dc.identifier.doi10.1128/JCM.00461-17
dc.identifier.issn0095-1137
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/51441
dc.identifier.wosWOS:000408109800019
dc.language.isoeng
dc.publisherAmer Soc Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTrichosporonen
dc.subjectCutaneotrichosporonen
dc.subjectApiotrichumen
dc.subjectVitek Men
dc.subjectMALDI-TOF mass spectrometryen
dc.titleEvaluating and Improving Vitek MS for Identification of Clinically Relevant Species of Trichosporon and the Closely Related Genera Cutaneotrichosporon and Apiotrichumen
dc.typeinfo:eu-repo/semantics/article
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