Effect of 830nm Laser Phototherapy on Osteoblasts Grown in Vitro on Biosilicate (R) Scaffolds

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dc.contributor.authorRenno, Ana Claudia Muniz [UNIFESP]
dc.contributor.authorMcDonnell, Pauline Ann
dc.contributor.authorCrovace, Murilo Camuri
dc.contributor.authorZanotto, Edgar Dutra
dc.contributor.authorLaakso, Liisa
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionGriffith Univ
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.date.accessioned2016-01-24T13:59:17Z
dc.date.available2016-01-24T13:59:17Z
dc.date.issued2010-02-01
dc.description.abstractObjective: the purpose of this study was (i) to develop a method for successfully seeding osteoblasts onto a glass-ceramic scaffold designed for use in clinical settings, and (ii) to determine whether the application of laser phototherapy at 830nm would result in osteoblast proliferation on the glass-ceramic scaffold. Background: the use of bioscaffolds is considered a promising strategy for a number of clinical applications where tissue healing is sub-optimal. As in vitro osteoblast growth is a slow process, laser phototherapy could be used to stimulate osteoblast proliferation on bioscaffolds. Methods: A methodology was developed to seed an osteoblastic (MC3T3) cell line onto a novel glass-ceramic scaffold. Seeded scaffolds were irradiated with a single exposure of 830nm laser at 10J/cm(2) (at diode). Non-irradiated seeded scaffolds acted as negative controls. Cell proliferation was assessed seven days after irradiation. Results: Osteoblastic MC3T3 cells were successfully grown on discs composed of a glass-ceramic composite. Laser irradiation produced a 13% decrease in MC3T3 cell proliferation on glass-ceramic discs (mean +/- SD 0.192 +/- 0.002) compared with control (non-irradiated) discs (mean +/- SD = 0.22 +/- 0.002). Conclusions: Despite successful seeding of bioscaffolds with osteoblasts, laser phototherapy resulted in a reduction in cell growth compared to non-irradiated controls. Future research combining laser phototherapy and glass-ceramic scaffolds should take into account possible interactions of the laser with matrix compounds.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biosci, BR-15011000 Santos, SP, Brazil
dc.description.affiliationGriffith Univ, Sch Biomol & Phys Sci, Nathan, Qld 4111, Australia
dc.description.affiliationUniv Fed Sao Carlos, Dept Mat Engn, Vitreous Mat Lab LaMaV, BR-13560 Sao Carlos, SP, Brazil
dc.description.affiliationGriffith Univ, Sch Physiotherapy & Exercise Sci, Gold Coast, Qld, Australia
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biosci, BR-15011000 Santos, SP, Brazil
dc.description.provenanceMade available in DSpace on 2016-01-24T13:59:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-02-01en
dc.description.sourceWeb of Science
dc.format.extent131-133
dc.identifierhttps://dx.doi.org/10.1089/pho.2009.2487
dc.identifier.citationPhotomedicine and Laser Surgery. New Rochelle: Mary Ann Liebert Inc, v. 28, n. 1, p. 131-133, 2010.
dc.identifier.doi10.1089/pho.2009.2487
dc.identifier.issn1549-5418
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/32245
dc.identifier.wosWOS:000274597500020
dc.language.isoeng
dc.publisherMary Ann Liebert Inc
dc.relation.ispartofPhotomedicine and Laser Surgery
dc.rightsAcesso restrito
dc.titleEffect of 830nm Laser Phototherapy on Osteoblasts Grown in Vitro on Biosilicate (R) Scaffoldsen
dc.typeArtigo
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