Influence of N-methyl-D-aspartate receptors on ouabain activation of nuclear factor-kappa B in the rat hippocampus

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dc.contributor.authorKawamoto, Elisa Mitiko
dc.contributor.authorLima, Larissa de Sá
dc.contributor.authorMunhoz, Carolina Demarchi
dc.contributor.authorYshii, Lidia Mitiko
dc.contributor.authorKinoshita, Paula Fernanda
dc.contributor.authorAmaral, Fernanda Gaspar
dc.contributor.authorPestana, Rafaela R. Ferrão
dc.contributor.authorOrellana, Ana Maria Marques
dc.contributor.authorCipolla-Neto, Jose
dc.contributor.authorBritto, Luiz Roberto Giorgetti de
dc.contributor.authorAvellar, Maria Christina Werneck [UNIFESP]
dc.contributor.authorRossoni, Luciana Venturini
dc.contributor.authorScavone, Cristoforo
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:17:41Z
dc.date.available2016-01-24T14:17:41Z
dc.date.issued2012-01-01
dc.description.abstractIt has been shown that ouabain (OUA) can activate the Na,K-ATPase complex and mediate intracellular signaling in the central nervous system (CNS). Inflammatory stimulus increases glutamatergic transmission, especially at N-methyl-D-aspartate (NMDA) receptors, which are usually coupled to the activation of nitric oxide synthase (NOS). Nuclear factor-kappa B (NF-kappa B) activation modulates the expression of genes involved in development, plasticity, and inflammation. the present work investigated the effects of OUA on NF-kappa B binding activity in rat hippocampus and the influence of this OUA-Na,K-ATPase signaling cascade in NMDA-mediated NF-kappa B activation. the findings presented here are the first report indicating that intrahippocampal administration of OUA, in a concentration that did not alter Na,K-ATPase or NOS activity, induced an activation of NF-kappa B, leading to increases in brain-derived neurotrophic factor (Bdnf), inducible NOS (iNos), tumor necrosis factor-alpha (Tnf-alpha), and B-cell leukemia/lymphoma 2 (Bcl2) mRNA levels. This response was not linked to any significant signs of neurodegeneration as showed via Fluoro-Jade B and Nissl stain. Intrahippocampal administration of NMDA induced NF alpha B activation and increased NOS and alpha 2/3-Na,K-ATPase activities. NMDA treatment further increased OUA-induced NF-kappa B activation, which was partially blocked by MK-801, an antagonist of NMDA receptor. These results suggest that OUA-induced NF-kappa B activation is at least in part dependent on Na,K-ATPase modulatory action of NMDA receptor in hippocampus. the interaction of these signaling pathways could be associated with biological mechanisms that may underlie the basal homeostatic state linked to the inflammatory signaling cascade in the brain. (c) 2011 Wiley Periodicals, Inc.en
dc.description.affiliationUniv São Paulo, Dept Pharmacol, Inst Biomed Sci, Mol Neuropharmacol Lab, BR-05508900 São Paulo, Brazil
dc.description.affiliationNIA, Neurosci Lab, NIH, Baltimore, MD 21224 USA
dc.description.affiliationUniv São Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.description.sponsorshipUniversidade de São Paulo
dc.description.sponsorshipIDFAPESP: 2003/08989-0pt
dc.description.sponsorshipIDCNPq: 485953-2007-2pt
dc.format.extent213-228
dc.identifierhttps://dx.doi.org/10.1002/jnr.22745
dc.identifier.citationJournal of Neuroscience Research. Malden: Wiley-Blackwell, v. 90, n. 1, p. 213-228, 2012.
dc.identifier.doi10.1002/jnr.22745
dc.identifier.issn0360-4012
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/34431
dc.identifier.wosWOS:000297951100019
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Neuroscience Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectGlutamateen
dc.subjectNF-kappa Ben
dc.subjectNaen
dc.subjectK-ATPaseen
dc.subjectN-methyl-D-aspartateen
dc.subjectOuabainen
dc.titleInfluence of N-methyl-D-aspartate receptors on ouabain activation of nuclear factor-kappa B in the rat hippocampusen
dc.typeinfo:eu-repo/semantics/article
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