In vitro growth and maturation of isolated caprine preantral follicles: Influence of insulin and FSH concentration, culture dish, coculture, and oocyte size on meiotic resumption

Página do item simplificado
dc.citation.volume90
dc.contributor.authorSilva, G. M.
dc.contributor.authorBrito, I. R.
dc.contributor.authorSales, A. D.
dc.contributor.authorAguiar, F. L. N.
dc.contributor.authorDuarte, A. B. G.
dc.contributor.authorAraujo, V. R.
dc.contributor.authorVieira, L. A.
dc.contributor.authorMagalhaes-Padilha, D. M.
dc.contributor.authorLima, L. F.
dc.contributor.authorAlves, B. G.
dc.contributor.authorSilveira, L. B. R.
dc.contributor.authorLo Turco, E. G.
dc.contributor.authorRodrigues, A. P.
dc.contributor.authorCampello, C. C.
dc.contributor.authorWheeler, M. B.
dc.contributor.authorFigueiredo, J. R.
dc.coverageNew York
dc.date.accessioned2020-07-17T14:02:54Z
dc.date.available2020-07-17T14:02:54Z
dc.date.issued2017
dc.description.abstractThe aims of this study were: (1) to evaluate the effect of different insulin concentrations, alone or in combination with either a fixed FSH concentration or increasing FSH concentrations on the in vitro culture of isolated caprine preantral follicles and (2) to analyze the efficiency of two IVM media and maturation culture systems (with or without coculture with in vivo grown oocytes) on the meiosis resumption. Secondary follicles were cultured for 18 days in a basic medium supplemented with low- or high-insulin concentration alone or with a fixed FSH concentration or with increasing FSH concentrations. Oocytes grown in vivo or in vitro were matured alone or cocultured. The high-insulin concentration associated with fixed FSH treatment had higher meiotic resumption rate (P < 0.05) and was the only treatment capable of producing oocytes in metaphase II. The rates of germinal vesicle, germinal vesicle breakdown, metaphase I, metaphase II (MII), meiotic resumption, and oocyte diameter were similar between the maturation media. In conclusion, a basic medium supplemented with 10-mu g/mL insulin and 100-mu g/mL FSH throughout the culture period improved meiotic resumption rate and produced MII oocytes from caprine pre antral follicles cultured in vitro. The MII rate was similar between in vivo and in vitro grown oocytes >110 mu m. (C) 2016 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniv Estadual Ceara, Lab Manipulat Oocyte & Preantral Follicles LAMOFO, Fac Vet Med, Fortaleza, Ceara, Brazil
dc.description.affiliationPotiguar Univ Laureate Int Univ, Postgrad Biotechnol, Natal, RN, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Div Urol, Human Reprod Sect, Dept Surg, Sao Paulo, Brazil
dc.description.affiliationUniv Illinois, Dept Anim Sci, 328 Mumford Hall, Urbana, IL 61801 USA
dc.description.affiliationUnifespUniv Fed Sao Paulo, Div Urol, Human Reprod Sect, Dept Surg, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.description.sponsorshipBiotecnologia-Rede Nordeste em Biotecnologia (RENORBIO)
dc.description.sponsorshipCoordenacan de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) Brazil
dc.description.sponsorshipIDRENORBIO: 554812/2006-1
dc.format.extent32-41
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2016.10.026
dc.identifier.citationTheriogenology. New York, v. 90, p. 32-41, 2017.
dc.identifier.doi10.1016/j.theriogenology.2016.10.026
dc.identifier.issn0093-691X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55078
dc.identifier.wosWOS:000394195400005
dc.language.isoeng
dc.publisherElsevier Science Inc
dc.relation.ispartofTheriogenology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectEmbryo corralen
dc.subjectOocyte competenceen
dc.subjectSecondary folliclesen
dc.titleIn vitro growth and maturation of isolated caprine preantral follicles: Influence of insulin and FSH concentration, culture dish, coculture, and oocyte size on meiotic resumptionen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos