Use of human serum for human corneal endothelial cell culture

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dc.contributor.authorVianna, Lucas Monferrari Monteiro [UNIFESP]
dc.contributor.authorKallay, Laura
dc.contributor.authorToyono, Tetsuya
dc.contributor.authorBelfort, Rubens [UNIFESP]
dc.contributor.authorHoliman, Jeffrey D.
dc.contributor.authorJun, Albert S.
dc.contributor.institutionJohns Hopkins Sch Med
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade do Estado do Rio de Janeiro (UERJ)
dc.contributor.institutionLions VisionGift
dc.date.accessioned2016-01-24T14:40:02Z
dc.date.available2016-01-24T14:40:02Z
dc.date.issued2015-02-01
dc.description.abstractBackground/aims To study human corneal endothelial cells (HCECs) cultured in vitro with human serum (HS) supplemented media (HS-SM) compared with HCEC cultured in fetal bovine serum (FBS) supplemented media (FBS-SM).Methods One cornea from a donor aged 21 years and a pair of corneas from a 16 year-old donor were obtained from the eye bank and used to create two different cell populations. At the first passage, the cell populations were equally divided and seeded in two different wells containing FBS-SM or HS-SM. in subsequent passages, HS-SM was compared with FBS-SM by morphology, growth curves, immunohistochemistry and real-time reverse-transcriptase PCR for endothelial cell markers.Results No difference in morphology could be seen in P2, P5 or in any other passages for cells grown in the two media. By growth curves, cell counts were similar in FBS-SM and HS-SM from days 1 to 5, with a trend towards higher cell counts in HS-SM at day 7. Cells grown in FBS-SM and HS-SM media showed similar expression of endothelial cell markers when assessed by immunohistochemistry and real-time reverse-transcriptase PCR.Conclusions HS-SM was similar to FBS-SM for HCEC culture when assessed by cell morphology, proliferation and protein/gene expression.en
dc.description.affiliationJohns Hopkins Sch Med, Dept Ophthalmol, Cornea & Anterior Segment Serv, Wilmer Eye Inst, Baltimore, MD 21231 USA
dc.description.affiliationUniversidade Federal de São Paulo, Paulista Sch Med, Dept Ophthalmol, São Paulo, Brazil
dc.description.affiliationUniv Estado Rio de Janeiro, Rio de Janeiro, Brazil
dc.description.affiliationLions VisionGift, Portland, OR USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Paulista Sch Med, Dept Ophthalmol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipJ Willard and Alice S Marriott Foundation
dc.description.sponsorshipNational Eye Institute
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipState University of Rio de Janeiro
dc.description.sponsorshipIDNational Eye Institute: EY001765
dc.format.extent267-271
dc.identifierhttp://dx.doi.org/10.1136/bjophthalmol-2014-306034
dc.identifier.citationBritish Journal of Ophthalmology. London: Bmj Publishing Group, v. 99, n. 2, p. 267-271, 2015.
dc.identifier.doi10.1136/bjophthalmol-2014-306034
dc.identifier.issn0007-1161
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38732
dc.identifier.wosWOS:000348285500026
dc.language.isoeng
dc.publisherBmj Publishing Group
dc.relation.ispartofBritish Journal of Ophthalmology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.titleUse of human serum for human corneal endothelial cell cultureen
dc.typeinfo:eu-repo/semantics/article
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