The chemical profile of senna velutina leaves and their antioxidant and cytotoxic effects

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dc.contributor.authorCampos, Jaqueline Ferreira
dc.contributor.authorHiramatsu de Castro, David Tsuyoshi
dc.contributor.authorDamiao, Marcio Jose
dc.contributor.authorVieira Torquato, Heron F. [UNIFESP]
dc.contributor.authorParedes-Gamero, Edgar J. [UNIFESP]
dc.contributor.authorCarollo, Carlos Alexandre
dc.contributor.authorEstevinho, Leticia M.
dc.contributor.authorSouza, Kely de Picoli
dc.contributor.authordos Santos, Edson Lucas
dc.date.accessioned2019-01-21T10:29:45Z
dc.date.available2019-01-21T10:29:45Z
dc.date.issued2016
dc.description.abstractNatural products can be a source of biomolecules with antioxidant activity which are able to prevent oxidative stress-induced diseases and show antitumor activity, making them important sources of new anticancer drug prototypes. In this context, this study aimed to analyze the chemical composition of an ethanol extract of Senna velutina leaves and to assess its antioxidant and cytotoxic activities in leukemic cells. The antioxidant properties were evaluated using a DPPH free radical scavenging assay and by examining the extract's inhibition of AAPH-induced lipid peroxidation in human erythrocytes. Its cytotoxicity and possible mechanisms of action were assessed in Jurkat and K562 leukemic cell lines. The ethanol extract contained flavonoids, such as epigallocatechin, epicatechin, kaempferol heteroside, rutin, and dimeric and trimeric proanthocyanidin derivatives. The extract exhibited antioxidant activity by scavenging free radicals and antihemolytic action, and it decreased malondialdehyde content in human erythrocytes. Furthermore, the extract also induced leukemic cell death by activating intracellular calcium and caspase-3, decreasing mitochondrial membrane potential, and arresting the cell cycle in S and G2 phases. Hence, S. velutina leaf extract contains antioxidant and antileukemic biomolecules with potential applications in diseases associated with oxidative stress and in the inhibition of tumor cell proliferation.en
dc.description.affiliationSchool of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Itahum, Km 12, 79.804-970 Dourados, MS, Brazil
dc.description.affiliationDepartment of Biochemistry, Federal University of São Paulo, Rua Pedro de Toledo 669, 04039-032 São Paulo, SP, Brazil
dc.description.affiliationInterdisciplinary Center of Biochemistry Investigation, University of Mogi das Cruzes, Av. Dr. Cândido Xavier de Almeida Souza, No. 200, Mogi das Cruzes, SP, Brazil
dc.description.affiliationCenter of Biological and Health Sciences, Federal University of Mato Grosso do Sul, Campo Grande, MS, Brazil
dc.description.affiliationDepartment of Biology and Biotechnology, Agricultural College of Bragança, Polytechnic Institute of Bragança, Campus Santa Apolónia, 5301-855 Bragança, Portugal
dc.description.affiliationUnifespDepartment of Biochemistry, Federal University of São Paulo, Rua Pedro de Toledo 669, 04039-032 São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Apoio ao Desenvolvimento do Ensino, Ciencia e Tecnologia do Mato Grosso do Sul (FUNDECT)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.description.sponsorshipBrazilian National Research Council (CNPq), Brazil
dc.identifierhttp://dx.doi.org/10.1155/2016/8405957
dc.identifier.citationOxidative Medicine And Cellular Longevity. London, 2016.
dc.identifier.doi10.1155/2016/8405957
dc.identifier.fileWOS000386287900001.pdf
dc.identifier.issn1942-0900
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/49375
dc.identifier.wosWOS:000386287900001
dc.language.isoeng
dc.publisherHospital Clinicas, Univ Sao Paulo
dc.relation.ispartofOxidative Medicine And Cellular Longevity
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCell-Cycle Progressionen
dc.subjectPhenolic-Compoundsen
dc.subjectFree-Radicalsen
dc.subjectIn-Vitroen
dc.subjectCanceren
dc.subjectProanthocyanidinsen
dc.subjectExtracten
dc.subjectFlavonoidsen
dc.subjectInductionen
dc.subjectApoptosisen
dc.titleThe chemical profile of senna velutina leaves and their antioxidant and cytotoxic effectsen
dc.typeinfo:eu-repo/semantics/article
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