Circulating levels of sTNFR and discrepancy between cytotoxicity and immunoreactivity of TNF-alpha in patients with visceral leishmaniasis

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dc.contributor.authorMedeiros, Iara Marques de [UNIFESP]
dc.contributor.authorReed, S.
dc.contributor.authorCastelo Filho, Adauto [UNIFESP]
dc.contributor.authorSalomão, Reinaldo [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Fed Rio Grande Norte
dc.contributor.institutionInfect Dis Res Inst
dc.date.accessioned2016-01-24T12:30:57Z
dc.date.available2016-01-24T12:30:57Z
dc.date.issued2000-01-01
dc.description.abstractObjective To study the influence of soluble tumour necrosis factor (TNF) receptors (sTNFR) on bioactivity; and immunoreactivity of TNF-alpha in patients with visceral leishmaniasis (Kala-azar) and to examine the association between circulating levels of sTNFR type I and type IT with clinical manifestations of the disease.Methods Ten patients with Kala-azar were enrolled. Plasma samples for TNF-alpha and sTNFR were obtained on days 0, 7 and 21-28 of antimonial therapy. Bioactivity of TNF-alpha was measured by cytotoxicity to L-929 cells and immunoreactivity by enzyme-linked immunosorbent assay (ELISA). sTNFR-I and sTNFR-II were measured by ELISA.Results Measured by ELISA, TNF-alpha was detected at baseline in all patients (range from 22.3 to 163 pg/mL) and showed a linear decline over time on therapy (r = -0.49, P = 0.007). in contrast, when measured by cytotoxicity assay, TNF-alpha was detected in only one patient at baseline (193 pg/mL) and in four patients at the end of therapy (38.7, 95, 133 and 232 pg/mL) and there was no linear association between TNF-alpha. and duration of therapy (r = -0.18, P = 0.45). sTNFR-I and sTNFR-II were detected in all patients before therapy. There was a strong positive correlation between plasma concentrations of sTNFR-I and sTNFR-II (r = 0.8, P = 0.006). Levels of sTNFR-I and sTNFR-II declined exponentially with time on therapyConclusions We concluded that sTNFR-I and sTNFR-II are related to disease activity in patients with Kala-azar and that these circulating receptors may interfere with the biological activity of TNF-alpha in patients with Kala-azar.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Div Infect Dis, UNIFESP, BR-04023900 São Paulo, SP, Brazil
dc.description.affiliationUniv Fed Rio Grande Norte, BR-59072970 Natal, RN, Brazil
dc.description.affiliationInfect Dis Res Inst, Seattle, WA USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Div Infect Dis, UNIFESP, BR-04023900 São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent34-37
dc.identifierhttp://dx.doi.org/10.1046/j.1469-0691.2000.00011.x
dc.identifier.citationClinical Microbiology and Infection. Oxford: Blackwell Science Ltd, v. 6, n. 1, p. 34-37, 2000.
dc.identifier.doi10.1046/j.1469-0691.2000.00011.x
dc.identifier.issn1198-743X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26202
dc.identifier.wosWOS:000086861000007
dc.language.isoeng
dc.publisherBlackwell Science Ltd
dc.relation.ispartofClinical Microbiology and Infection
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectvisceral leishmaniasisen
dc.subjecttumour necrosis factoren
dc.subjectsoluble tumour necrosis factor receptoren
dc.subjectsTNFRen
dc.titleCirculating levels of sTNFR and discrepancy between cytotoxicity and immunoreactivity of TNF-alpha in patients with visceral leishmaniasisen
dc.typeinfo:eu-repo/semantics/article
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