Predominance of CD4 Th1 and CD8 Tc1 cells revealed by characterization of the cellular immune response generated by immunization with a DNA vaccine containing a Trypanosoma cruzi gene

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dc.contributor.authorRodrigues, Mauricio Martins [UNIFESP]
dc.contributor.authorRibeirao, Marcelo [UNIFESP]
dc.contributor.authorPereira-Chioccola, Vera Lucia [UNIFESP]
dc.contributor.authorRenia, Laurent
dc.contributor.authorCosta, Fabio [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionInst Dante Pazzanese Cardiol Estado Sao Paulo
dc.contributor.institutionUniv Paris 05
dc.date.accessioned2018-06-15T17:38:39Z
dc.date.available2018-06-15T17:38:39Z
dc.date.issued1999-08-01
dc.description.abstractImmunization with a plasmid DNA containing the gene encoding the catalytic domain of trans-sialidase (TS) elicits protective immune responses against experimental Trypanosoma cruzi infection. As several studies provided strong evidence that during infection CD4 Th1 and CD8 T cytotoxic type 1 (Tc1) cells are important factors in host resistance, the present study was designed to evaluate which T-cell types were activated in DNA-vaccinated BALB/c mice. We found that bulk cells from DNA-immunized mice had CD4 and CD8 T cells that produced gamma interferon (IFN-gamma) but not interleukin-4 (IL-4) or IL-10. To characterize the TS-specific T cells at the clonal level, we generated CD4 and CD8 clones. We obtained cytotoxic CD4 clones of the Th1 type that secreted large amounts of IFN-gamma but not IL-4 or IL-10. Unexpectedly, we obtained other CD4 clones with a Th2 phenotype, secreting IL-4 and IL-10 but not IFN-gamma. All CD8 clones were cytotoxic and produced IFN-gamma. IL-4 and IL-10 were not secreted by these cells. Using synthetic peptides, we determined a CDS epitope recognized by several clones as being represented by amino acids IYNVGQVSI. The antiparasitic activity of a CD4 Th1 and a CDS Tc1 clone was assessed in vitro. CD4 or CD8 T cells significantly inhibited T. cruzi development in infected macrophages or fibroblasts, respectively. We concluded that DNA vaccine efficiently generates potentially protective CD4 Th1 and CD8 Tc1 cells specific for a T. cruzi antigen, therefore reinforcing the possibility of using this strategy for developing a preventive or therapeutic vaccine against Chagas' disease.en
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo, Brazil
dc.description.affiliationInst Dante Pazzanese Cardiol Estado Sao Paulo, Lab Xenodiagnost, Sao Paulo, Brazil
dc.description.affiliationUniv Paris 05, Hop Cochin 27, Inst Cochin Genet Mol,Lab Immunol Pathol Infect &, INSERM,U445, F-75014 Paris, France
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent3855-3863
dc.identifierhttp://iai.asm.org/content/67/8/3855.abstract
dc.identifier.citationInfection And Immunity. Washington: Amer Soc Microbiology, v. 67, n. 8, p. 3855-3863, 1999.
dc.identifier.issn0019-9567
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/43968
dc.identifier.wosWOS:000081637400023
dc.language.isoeng
dc.publisherAmer Soc Microbiology
dc.relation.ispartofInfection And Immunity
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.titlePredominance of CD4 Th1 and CD8 Tc1 cells revealed by characterization of the cellular immune response generated by immunization with a DNA vaccine containing a Trypanosoma cruzi geneen
dc.typeinfo:eu-repo/semantics/article
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