Identification of transcription elements in the 5 ' intergenic region shared by LON and MDJ1 heat shock genes from the human pathogen Paracoccidioides brasiliensis. Evaluation of gene expression

dc.contributor.authorBatista, Wagner L.
dc.contributor.authorBarros, Tania F.
dc.contributor.authorGoldman, Gustavo H.
dc.contributor.authorMorais, Flavia V.
dc.contributor.authorPuccia, Rosana
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal da Bahia (UFBA)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniv Vale Paraiba
dc.date.accessioned2016-01-24T13:48:39Z
dc.date.available2016-01-24T13:48:39Z
dc.date.issued2007-05-01
dc.description.abstractThe MDJ1ILON locus is conserved among pathogenic dimorphic fungi. We have mapped using DNase I footprinting and mobility shift assays three putative heat shock elements and one AP-1 binding domain (ARE) in the 5' intergenic region shared by PbMDJ1 and PbLON (ML) from Paracoccidioides brasiliensis. the region bearing an ARE-like towards PbLON also has an opposite skn-1-like element. We studied genetically and pathogenically distinct isolates Pb18 and Pb3, where ML is polymorphic and the number of elements detected was higher. the functionality of the elements was suggested by the stimulatory response of both genes to beat shock and oxidative stress. Co-regulation occurred upon heat shock from 36 to 42 degrees C and, only in Pb3, also during mycelium to yeast transformation (26-36 degrees C). in Pb18, PbMDJ1 seemed to be preferentially expressed in yeast. Our study might help understand regulation of genes involved in fungal adaptation to the host. (c) 2006 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUniv Fed Bahia, Dept Anal Clin & Toxol, BR-41170290 Salvador, BA, Brazil
dc.description.affiliationUniv São Paulo, Fac Ciencias Farmaceut Ribeirao Preto, São Paulo, Brazil
dc.description.affiliationUniv Vale Paraiba, Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent347-356
dc.identifierhttp://dx.doi.org/10.1016/j.fgb.2006.11.002
dc.identifier.citationFungal Genetics and Biology. San Diego: Academic Press Inc Elsevier Science, v. 44, n. 5, p. 347-356, 2007.
dc.identifier.doi10.1016/j.fgb.2006.11.002
dc.identifier.issn1087-1845
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/29702
dc.identifier.wosWOS:000245643100006
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofFungal Genetics and Biology
dc.rightsAcesso restrito
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectParacoccidioides brasiliensisen
dc.subjectMDJ1en
dc.subjectLONen
dc.subjecttranscription elementsen
dc.subjectgene expressionen
dc.subjectheat shocken
dc.subjectoxidative stressen
dc.titleIdentification of transcription elements in the 5 ' intergenic region shared by LON and MDJ1 heat shock genes from the human pathogen Paracoccidioides brasiliensis. Evaluation of gene expressionen
dc.typeArtigo
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