Expression of the selectable marker gene bsrm in BALB/MK cells induces apoptosis by overproduction of hydrogen peroxide

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dc.contributor.authorTakeshita, Daniela
dc.contributor.authorBento, Fernanda Mara
dc.contributor.authorChammas, Roger
dc.contributor.authorBelizario, Jose Ernesto
dc.contributor.authorCarmona, Adriana Karaoglanovic
dc.contributor.authorKonno, Katsuhiro
dc.contributor.authorMelo, Robson Lopes de
dc.contributor.authorMolina, Gustavo
dc.contributor.authorLisboa, Bianca Cristina Garcia
dc.contributor.authorHan, Sang Won [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionButantan Inst
dc.date.accessioned2016-01-24T13:49:06Z
dc.date.available2016-01-24T13:49:06Z
dc.date.issued2007-10-01
dc.description.abstractTransduction of the retroviral vector LBmSN, which expresses the blasticidin S resistance gene bsrm in the murine keratinocyte cell line BALB/MK, induces death in these cells. Cell death is caused by a factor called DOKEB (death factor obtained from keratinocytes expressing bsrm), which is released before the cells' death. in this report we describe and discuss the purification and characterization of DOKEB. Our results were as follows. (i) the 5-day-old medium from the modified BALB/MK cells with LBmSN was used for purification and characterization by filtration and chromatography: DOKEB was a stable and highly hydrophilic compound, with a molecular mass less than that of I amino acid. (ii) the conditioned medium containing DOKEB was reactive against thiobarbituric acid and dichlorofluorescein diacetate. (iii) DOKEB activity was neutralized by the incubation of the conditioned medium with catalase. Therefore, our conclusion is that the BALB/MK cells expressing bsrm produce a large amount of hydrogen peroxide, which catalyzes the process of apoptosis of those cells.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biophys, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Interdisciplinary Ctr Gene Therapy, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med, Dept Radiol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, ICB, Dept Pharmacol, BR-09500900 São Paulo, Brazil
dc.description.affiliationButantan Inst, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biophys, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Interdisciplinary Ctr Gene Therapy, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent573-581
dc.identifierhttp://dx.doi.org/10.1139/O07-058
dc.identifier.citationBiochemistry and Cell Biology-biochimie Et Biologie Cellulaire. Ottawa: Natl Research Council Canada-n R C Research Press, v. 85, n. 5, p. 573-581, 2007.
dc.identifier.doi10.1139/O07-058
dc.identifier.issn0829-8211
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/30058
dc.identifier.wosWOS:000250624500005
dc.language.isoeng
dc.publisherNatl Research Council Canada-n R C Research Press
dc.relation.ispartofBiochemistry and Cell Biology-biochimie Et Biologie Cellulaire
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectselectable markeren
dc.subjectapoptosisen
dc.subjecthydrogen peroxideen
dc.subjectblasticidin S.en
dc.titleExpression of the selectable marker gene bsrm in BALB/MK cells induces apoptosis by overproduction of hydrogen peroxideen
dc.typeinfo:eu-repo/semantics/article
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