Serotonin concentration, synthesis, cell origin, and targets in the rat caput epididymis during sexual maturation and variations associated with adult mating status: Morphological and biochemical studies

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dc.contributor.authorJimenez-Trejo, Francisco
dc.contributor.authorTapia-Rodriguez, Miguel
dc.contributor.authorQueiroz, Daniel Barboza Cava [UNIFESP]
dc.contributor.authorPadilla, Patricia
dc.contributor.authorAvellar, Maria Christina Werneck [UNIFESP]
dc.contributor.authorManzano, Patricia Rivas
dc.contributor.authorManjarrez-Gutierrez, Gabriel
dc.contributor.authorGutierrez-Ospina, Gabriel
dc.contributor.institutionNatl Autonomous Univ Mexico
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionXXI Century Natl Med Ctr
dc.date.accessioned2016-01-24T12:41:42Z
dc.date.available2016-01-24T12:41:42Z
dc.date.issued2007-01-01
dc.description.abstractThe caput epididymis of some mammals contains large quantities of serotonin whose origin, targets, and physiological variations have been poorly studied. We combined morphological and biochemical techniques to begin approaching these aspects of serotonin in the rat caput epididymis. Serotonin immunostaining was detected in mast, epithelial, and neuroendocrine cells. Epithelial cells displayed immunoreactivity to 5HT(1A), 5HT(2A), and 5HT(3) serotonin receptors. Endothelial and mast cells labeled positive for 5HT(1B) serotonin receptors and spermatozoa displayed 5HT2A and 5HT3 serotonin receptor immunoreactivity. Epithelial, endothelial, and mast cells stained positive for serotonin transporters. Only epithelial cells showed tryptophan hydroxylase immunoreactivity; this enzyme catalyzes the limiting step in the serotonin synthetic pathway. in addition, Western blot analyses of caput homogenates documented the presence of 2 protein bands (approximate to 51 kd and 48 kd) that were immunoreactive for tryptophan hydroxylase. Chromatographic analyses documented the presence of tryptophan hydroxylase in the caput, and showed that both its activity and serotonin availability increased with sexual maturation and decreased following p-chlorophenylalanine treatment, an inhibitor of tryptophan hydroxylase activity. Interestingly, serotonin concentration and tryptophan hydroxylase activity tended to be higher in breeding males than in those with no mating experience. We think that these results support the existence of a local serotoninergic system in the rat caput epididymis that might regulate some aspects of male reproductive function.en
dc.description.affiliationNatl Autonomous Univ Mexico, Biomed Res Inst, Dept Cell Biol & Physiol, Mexico City 04510, DF, Mexico
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol, São Paulo, Brazil
dc.description.affiliationNatl Autonomous Univ Mexico, Fac Sci, Dept Reprod Biol, Mexico City 04510, DF, Mexico
dc.description.affiliationXXI Century Natl Med Ctr, Unit Med Invest Neurol Dis, Mexico City, DF, Mexico
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent136-149
dc.identifierhttp://dx.doi.org/10.2164/jandrol.106.000653
dc.identifier.citationJournal of Andrology. Lawrence: Amer Soc Andrology, Inc, v. 28, n. 1, p. 136-149, 2007.
dc.identifier.doi10.2164/jandrol.106.000653
dc.identifier.issn0196-3635
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/29349
dc.identifier.wosWOS:000243141400018
dc.language.isoeng
dc.publisherAmer Soc Andrology, Inc
dc.relation.ispartofJournal of Andrology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject5-hydroxytryptamineen
dc.subjecttryptophan hydroxylaseen
dc.subjectneuroendocrine cellsen
dc.subjectmale reproductionen
dc.subjectserotonin receptoren
dc.subjectspermatozoaen
dc.titleSerotonin concentration, synthesis, cell origin, and targets in the rat caput epididymis during sexual maturation and variations associated with adult mating status: Morphological and biochemical studiesen
dc.typeinfo:eu-repo/semantics/article
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