Antibodies to human neutrophil antigen HNA-3b implicated in cases of neonatal alloimmune neutropenia

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dc.citation.issue5
dc.citation.volumev. 58
dc.contributor.authorLopes, Larissa Barbosa [UNIFESP]
dc.contributor.authorAbbas, Samira Ali [UNIFESP]
dc.contributor.authorMoritz, Elyse [UNIFESP]
dc.contributor.authorMartins, Juliana Oliveira [UNIFESP]
dc.contributor.authorChiba, Akemi Kuroda [UNIFESP]
dc.contributor.authorLanghi Júnior, Dante Mário [UNIFESP]
dc.contributor.authorBordin, Jose Orlando [UNIFESP]
dc.coverageHoboken
dc.date.accessioned2020-07-20T16:30:54Z
dc.date.available2020-07-20T16:30:54Z
dc.date.issued2018
dc.description.abstractBACKGROUNDNeonatal alloimmune neutropenia results from maternal alloimmunization to human neutrophil antigens. The alloantibodies involved in neonatal alloimmune neutropenia are against human neutrophil antigens HNA-1a, HNA-1b, HNA-1c, HNA-1d, HNA-2, HNA-3a, HNA-4a, HNA-4b, and HNA-5aen
dc.description.abstracthowever, to date, antibodies specific to HNA-3b have not been reported. STUDY DESIGN AND METHODSBlood samples from 10,000 unselected neonates were analyzed, resulting in the selection of 88 neutropenic newborns (neutrophil count <1.5 x 10(9)/L) from 83 mothers (three pairs of twins and one triplet). HNA-3 genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism to identify the cases of maternal-fetal HNA-3 incompatibility. Serologic studies for detecting maternal HNA-3 alloantibodies were performed with the granulocyte agglutination test, the white blood cell immunofluorescence test, and a LABScreen Multi-HNA Kit. RESULTSGenotyping studies identified 13 of 88 (14.8%) instances of maternal-fetal HNA-3 incompatibility, with all mothers typed as HNA-3a/a and neonates typed as HNA-3a/b. Serologic studies revealed that five of 13 (38.5%) mothers carried anti-HNA-3b plus human leukocyte antigen antibodies and that three of 13 (23.1%) mothers had anti-HNA-3b without human leukocyte antigen antibodies. CONCLUSIONHere, we report the first three cases of neonatal alloimmune neutropenia associated with HNA-3b antibodies resulting in a neonatal alloimmune neutropenia incidence of one in 3333 live births.en
dc.description.affiliationUniv Fed Sao Paulo, UNIFESP, Disciplina Hematol & Hemoterapia, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, UNIFESP, Disciplina Hematol & Hemoterapia, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) "Doctoral Fellowship"
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) "Edital Universal"
dc.description.sponsorshipCoordenacao de Aperfeicoamento Pessoal de Nivel Superior (CAPES)-"Programa Institucional de Doutorado Sanduiche no Exterior"/PDSE
dc.description.sponsorshipCoordenacao de Aperfeicoamento Pessoal de Nivel Superior (CAPES)-"Postdoctoral Fellowship"/PNPD
dc.description.sponsorshipIDCNPq: 142825/2009-3
dc.description.sponsorshipIDCNPq: 475806/2010-7
dc.description.sponsorshipIDCAPES: 7352/12-3
dc.format.extent1264-1270
dc.identifierhttp://dx.doi.org/10.1111/trf.14524
dc.identifier.citationTransfusion. Hoboken, v. 58, n. 5, p. 1264-1270, 2018.
dc.identifier.doi10.1111/trf.14524
dc.identifier.issn0041-1132
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55582
dc.identifier.wosWOS:000430691300025
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofTransfusion
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleAntibodies to human neutrophil antigen HNA-3b implicated in cases of neonatal alloimmune neutropeniaen
dc.typeinfo:eu-repo/semantics/article
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